In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
In situ gellable hydrogels are more attractive in many biomedical and biopharmaceutical applications than pre-formed hydrogels because they can be implanted simply by injection and allow homogeneous incorporation of bioactive materials. In this study, the potential suitability of in situ gellable sugar beet pectin (SBP) for biomedical and biopharmaceutical applications was investigated. SBP aqueous solution gelled within 1min after addition of appropriate amounts of horseradish peroxidase (HRP) and H_2O_2via HRP-catalyzed oxidative coupling reaction of feruloyl groups on SBP molecules. The resultant gels gradually degraded under simulated physiological condition. L929 fibroblast cells encapsulated in the gels were scarcely damaged during the gelation process. A subcutaneously injected mixture of SBP, HRP and H_2O_2 solutions successfully gelled, and the gel did not induce necrosis in the surrounding tissue 1 week after implantation. These results demonstrate that the in situ gellable SBP gels are useful for biomedical and biopharmaceutical applications.
著者
-
Takei Takayuki
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
-
Ijima Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
-
Kawakami Koei
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
-
Ijima Hiroyuki
Dep. Of Chemical Engineering Fac. Of Engineering Graduate School Kyushu Univ. 744 Motooka Nishi-ku F
-
Kawakami Koei
Department Of Chemical Engineering Graduate School Of Engineering Kyushu University
-
Sugihara Kotaro
Department of Chemical Engineering, Graduate School of Engineering, Kyushu University
-
Sugihara Kotaro
Department Of Chemical Engineering Graduate School Of Engineering Kyushu University
関連論文
- Heat treatment of electrospun silicate fiber substrates enhances cellular adhesion and proliferation(CELL AND TISSUE ENGINEERING)
- Preparation of cell-enclosing microcapsules through photopolymerization of methacrylated alginate solution triggered by irradiation with visible light(BIOCHEMICAL ENGINEERING)
- Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes(CELL AND TISSUE ENGINEERING)
- Lactic Acid Bacteria-Enclosing Poly (ε-Caprolactone) Microcapsules as Soil Bioamendment(ENVIRONMENTAL BIOTECHNOLOGY)
- Transesterification by Lipase Entrapped in Electrospun Poly(Vinyl Alcohol) Fibers and Its Application to a Flow-Through Reactor(BIOCHEMICAL ENGINEERING)
- Development of a hybrid artificial liver support system and preclinical animal experiments
- Enhancement of Cell Function through Heterotypic Cell-Cell Interactions Using E-Cadherin-Expressing NIH3T3 Cells(CELL AND TISSUE ENGINEERING)
- Albumin production activity of primary rat hepatocytes is improved on type V collagen(CELL AND TISSUE ENGINEERING)
- Enhancement of the Activity of Renatured Lysozyme by Protein Disulfide Isomerase(BIOCHEMICAL ENGINEERING)
- Novel Technique for Fabricating Double-Layered Tubular Constructs Consisting of Two Vascular Cell Types in Collagen Gels Used as Templates for Three-Dimensional Tissues(CELL AND TISSUE ENGINEERING)
- Expression of a Liver-Specific Function by a Hepatoblastoma Cell Line Cocultured with Three-Dimensional Endothelialized Tubes in Collagen Gels(CELL AND TISSUE ENGINEERING)
- Enhanced Angiogenesis in bFGF-Containing Scaffold Promoted Viability of Enclosed Hepatocytes and Maintained Hepatospecific Glycogen Storage Capacity
- Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces
- Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes(CELL AND TISSUE ENGINEERING)
- Hepatic Differentiation of Mouse Embryonic Stem Cells in a Three-Dimensional Culture System Using Polyurethane Foam(CELL AND TISSUE ENGINEERING)
- The Mixed Coculture Effect of Primary Rat Hepatocytes and Bone Marrow Cells Is Caused by Soluble Factors Derived from Bone Marrow Cells(CELL AND TISSUE ENGINEERING)
- Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science and Engineering)
- Promotion of Monolayer Formation and High Expression of Ammonia Metabolism of Primary Rat Hepatocytes on Arginine-Glycine-Aspartic Acid-Containing Peptide-Coated Polystyrene Dish(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science a
- Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period
- Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum : Use as an Animal Cell Culture Substratum
- Permeability of a sol-gel synthesized aminopropyl-silicate-titanate hybrid membrane for the microcapsule-shaped bioartificial pancreas
- Development of a Hybrid Artificial Liver Based on Hepatocyte Organoid Culture Technique for Chronic Liver Failure Patients
- Thermal Stabilization of Lipase by Sol-Gel Entrapment in Organically Modified Silicates Formed on Kieselguhr
- Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis
- Large-Scale Cultivation of Embryonic and Other Stem Cells, Induced Differentiation into Various Cell Types, and Development of Bio-artificial Organ Systems
- Synthesis of Enzymatically-Gellable Carboxymethylcellulose for Biomedical Applications(CELL AND TISSUE ENGINEERING)
- Synthesis of an Agarose-Gelatin Conjugate for Use as a Tissue Engineering Scaffold(CELL AND TISSUE ENGINEERING)
- Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis(CELL AND TISSUE ENGINEERING)
- In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications(CELL AND TISSUE ENGINEERING)
- Enzymatic Hydrolysis of Soluble Starch in a Polyethylene Glycol-Dextran Aqueous Two-Phase System
- Enhancement of Enzymatic Production of Cyclodextrins by Adding Polyethylene Glycol or Polypropylene Glycol
- Lactic Acid Bacteria-Enclosing Poly(ε-Caprolactone) Microcapsules as Soil Bioamendment
- Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering(CELL AND TISSUE ENGINEERING)
- Controlling the Diameters of Silica Nanofibers Obtained by Sol-Gel/Electrospinning Methods
- Controlling the Diameters of Silica Nanofibers Obtained by Sol–Gel/Electrospinning Methods
- Heparin-conjugated gelatin as a growth factor immobilization scaffold(CELL AND TISSUE ENGINEERING)
- Nucleus number in clusters of transplanted fetal liver cells increases by partial hepatectomy of recipient rats(CELL AND TISSUE ENGINEERING)
- The individual and competitive hydrogenations of ethylene and propylene in a stirred slurry reactor.
- Solubilized matrix derived from decellularized liver as a growth factor-immobilizable scaffold for hepatocyte culture(CELL AND TISSUE ENGINEERING)
- The effects of intraparticle diffusion on the yield of the liquid-phase hydrogenation of phenylacetylene in a stirred basket reactor.
- The effectiveness factor of a catalyst pellet in the liquid-phase hydrogenation of styrene.
- An empirical kinetic model and simulation for anodic oxidation of coal slurry.
- Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering
- Cesium accumulation of Rhodococcus erythropolis CS98 strain immobilized in hydrogel matrices(BIOCHEMICAL ENGINEERING)