Heparin-conjugated gelatin as a growth factor immobilization scaffold(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
Tissue engineering requires growth factors, cells and a scaffold to permit effective tissue regeneration. This study aimed to develop a scaffold with a focus on immobilizing growth factors within gelatin. We focused on the extracellular matrix and developed a heparin-conjugated gelatin (Hep-gela). Conjugation was confirmed using the alcian blue assay and X-ray diffraction patterns. The mechanical strength and stability of the Hep-gela gel in protease solution were improved compared with collagen gel. Hep-gela was able to immobilize vascular endothelial growth factor (VEGF) even in the presence of albumin, with an efficiency of 54.2%. Immobilized VEGF promoted proliferation of human umbilical vein endothelial cells. Hep-gela-immobilized VEGF maintained its native biological activity. In summacy, Hep-gela has the potential to become an effective material in the field of regenerative medicine.
- 2013-05-00
著者
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Kubo Takafumi
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
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Ijima Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
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Ijima Hiroyuki
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University
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Nakamura Shintaro
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University
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