Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
A hepatocyte growth factor (HGF)/heparin-immobilized collagen system was used as a synthetic extracellular matrix for hepatocyte culture. The albumin synthesis, nucleus numbers and morphology of the hepatocytes were determined separately to evaluate the hepatocyte number and hepatocyte-specific function under this system. The benefits of the HGF/heparin-immobilized collagen system for hepatocyte culture were confirmed by three types of culture methods in vitro, namely 2D film cultures, 2D gel cultures and 3D gel cultures. In 2D collagen film cultures, hepatocytes exhibited the highest albumin synthesis (1.42μg/well/day) in HGF/heparin-immobilized collagen films at 7 days of culture. Heparin inhibited hepatocyte adhesion while HGF promoted hepatocyte migration, and spheroid formation was easily detected in HGF/heparin-immobilized collagen films. In 2D collagen gel cultures, albumin synthesis of around 15μg/well/day was detected and maintained for more than 18 days on HGF/heparin-immobilized collagen gels. Similar findings were obtained in 3D HGF/heparin-immobilized collagen gel cultures, which exhibited albumin synthesis of up to 30μg/well/day. The albumin synthesis by hepatocytes was two-fold higher in 3D gel cultures compared with 2D gel cultures, and was maintained for over 2 weeks compared with 2D film cultures using the HGF/heparin-immobilized collagen system. Taken together, the HGF/heparin-immobilized collagen system was effective for albumin synthesis by hepatocytes in both 2D film cultures and 3D gel cultures, and therefore shows good potential for tissue engineering use.
著者
-
Sakai Shinji
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
-
Kawakami Koei
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
-
Takei Takayuki
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
-
Ijima Hiroyuki
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
-
Takei Takayuki
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
-
Hou Yung-Te
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University
-
Matsumoto Shunichi
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University
-
Kubo Takafumi
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University
-
Kubo Takafumi
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
-
Ijima Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
-
Matsumoto Shunichi
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
-
Matsumoto Shunichi
Department Of Applied Chemistry Nagoya Institute Of Technology
-
Hou Yung-te
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
-
Sakai Shinji
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
-
Kawakami Koei
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
-
Ijima Hiroyuki
Dep. Of Chemical Engineering Fac. Of Engineering Graduate School Kyushu Univ. 744 Motooka Nishi-ku F
-
Kawakami Koei
Department Of Chemical Engineering Graduate School Of Engineering Kyushu University
-
Sakai Shinji
Div. Of Chemical Engineering Dep. Of Materials Engineering Sci. Graduate School Of Engineering Sci. Osaka Univ.
関連論文
- Heat treatment of electrospun silicate fiber substrates enhances cellular adhesion and proliferation(CELL AND TISSUE ENGINEERING)
- Preparation of cell-enclosing microcapsules through photopolymerization of methacrylated alginate solution triggered by irradiation with visible light(BIOCHEMICAL ENGINEERING)
- Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes(CELL AND TISSUE ENGINEERING)
- Heat treatment of electrospun silicate fiber substrates enhances cellular adhesion and proliferation
- Lactic Acid Bacteria-Enclosing Poly (ε-Caprolactone) Microcapsules as Soil Bioamendment(ENVIRONMENTAL BIOTECHNOLOGY)
- Blackening of sugi (Cryptomeria japonica D.Don) heartwood in relation to metal content and moisture content
- Influence of osmotic pressure on somatic embryo maturation in Pinus densiflora
- Changes in levels of endogenous plant hormones in cambial regions of stems of Larix kaempferi at the onset of cambial activity in springtime
- Somatic embryogenesis from immature and mature zygotic embryos of Cryptomeria japonica l : Embryogenic cell induction and its morphological characteristics
- Transesterification by Lipase Entrapped in Electrospun Poly(Vinyl Alcohol) Fibers and Its Application to a Flow-Through Reactor(BIOCHEMICAL ENGINEERING)
- Development of a hybrid artificial liver support system and preclinical animal experiments
- Enhancement of Cell Function through Heterotypic Cell-Cell Interactions Using E-Cadherin-Expressing NIH3T3 Cells(CELL AND TISSUE ENGINEERING)
- Albumin production activity of primary rat hepatocytes is improved on type V collagen(CELL AND TISSUE ENGINEERING)
- Intra-arterial Digital Subtraction Angiography with Extra-large Field Using a Computed Radiography System in Evaluation Peripheral Vascular Disease
- Enhancement of the Activity of Renatured Lysozyme by Protein Disulfide Isomerase(BIOCHEMICAL ENGINEERING)
- Ring Hydroxylations of Aromatic Amino Acid Derivatives and Toluene by Hydrogen Peroxide Catalyzed by Manganese Halogenated Porphyrins in CH_2Cl_2/H_2O and Lipid Bilayers
- Novel Technique for Fabricating Double-Layered Tubular Constructs Consisting of Two Vascular Cell Types in Collagen Gels Used as Templates for Three-Dimensional Tissues(CELL AND TISSUE ENGINEERING)
- Expression of a Liver-Specific Function by a Hepatoblastoma Cell Line Cocultured with Three-Dimensional Endothelialized Tubes in Collagen Gels(CELL AND TISSUE ENGINEERING)
- Enhanced Angiogenesis in bFGF-Containing Scaffold Promoted Viability of Enclosed Hepatocytes and Maintained Hepatospecific Glycogen Storage Capacity
- Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces
- Dural Non-cavernous Sinus Arteriovenous Fistulas Symptomatically Simulating Spontaneous Carotid-carvrnous Fistulas: An Analysis of Angiographic Findings
- Intracranial Metastasis of Renal Cell Carcinoma : MR Imaging
- Physiological tolerance of Camellia rusticana leaves to heavy snowfall environments : The effects of prolonged snow cover on evergreen leaves
- Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes(CELL AND TISSUE ENGINEERING)
- Hepatic Differentiation of Mouse Embryonic Stem Cells in a Three-Dimensional Culture System Using Polyurethane Foam(CELL AND TISSUE ENGINEERING)
- MR Imaging of Idiopathic Trigeminal Neuralgia: Correlation with Non-Surgical Therapy
- Postcontrast 3D - TOF - MR Angiography of Intracranial Venous Angiomas
- The Optical Absorption in Cubic PbF_2 Crystal Induced with γ-Ray Irradiation at Liquid-Helium Temperature
- Usefulness of cumulative smoking dose for identifying the EGFR mutation and patients with non-small-cell lung cancer for gefitinib treatment
- The Mixed Coculture Effect of Primary Rat Hepatocytes and Bone Marrow Cells Is Caused by Soluble Factors Derived from Bone Marrow Cells(CELL AND TISSUE ENGINEERING)
- Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science and Engineering)
- Promotion of Monolayer Formation and High Expression of Ammonia Metabolism of Primary Rat Hepatocytes on Arginine-Glycine-Aspartic Acid-Containing Peptide-Coated Polystyrene Dish(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science a
- Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period
- Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum : Use as an Animal Cell Culture Substratum
- Permeability of a sol-gel synthesized aminopropyl-silicate-titanate hybrid membrane for the microcapsule-shaped bioartificial pancreas
- Development of a Hybrid Artificial Liver Based on Hepatocyte Organoid Culture Technique for Chronic Liver Failure Patients
- New Electrode-Wire CT Scanner for Void Distribution Measurements
- Thermal Stabilization of Lipase by Sol-Gel Entrapment in Organically Modified Silicates Formed on Kieselguhr
- Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis
- Large-Scale Cultivation of Embryonic and Other Stem Cells, Induced Differentiation into Various Cell Types, and Development of Bio-artificial Organ Systems
- Synthesis of Enzymatically-Gellable Carboxymethylcellulose for Biomedical Applications(CELL AND TISSUE ENGINEERING)
- Synthesis of an Agarose-Gelatin Conjugate for Use as a Tissue Engineering Scaffold(CELL AND TISSUE ENGINEERING)
- Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis(CELL AND TISSUE ENGINEERING)
- In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications(CELL AND TISSUE ENGINEERING)
- Enzymatic Hydrolysis of Soluble Starch in a Polyethylene Glycol-Dextran Aqueous Two-Phase System
- Enhancement of Enzymatic Production of Cyclodextrins by Adding Polyethylene Glycol or Polypropylene Glycol
- Clinical and microbiological evaluation of hemodialysis-associated pneumonia (HDAP) : should HDAP be included in healthcare-associated pneumonia?
- Lactic Acid Bacteria-Enclosing Poly(ε-Caprolactone) Microcapsules as Soil Bioamendment
- Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering(CELL AND TISSUE ENGINEERING)
- Controlling the Diameters of Silica Nanofibers Obtained by Sol-Gel/Electrospinning Methods
- Controlling the Diameters of Silica Nanofibers Obtained by Sol–Gel/Electrospinning Methods
- Enzymatically-gelled amylopectin-based substrates enable on-demand harvesting cells with preserving cell-to-cell connection using saliva(CELL AND TISSUE ENGINEERING)
- Heparin-conjugated gelatin as a growth factor immobilization scaffold(CELL AND TISSUE ENGINEERING)
- Nucleus number in clusters of transplanted fetal liver cells increases by partial hepatectomy of recipient rats(CELL AND TISSUE ENGINEERING)
- Fabrication of in vitro three-dimensional multilayered blood vessel model using human endothelial and smooth muscle cells and high-strength PEG hydrogel(CELL AND TISSUE ENGINEERING)
- The individual and competitive hydrogenations of ethylene and propylene in a stirred slurry reactor.
- Solubilized matrix derived from decellularized liver as a growth factor-immobilizable scaffold for hepatocyte culture(CELL AND TISSUE ENGINEERING)
- The effects of intraparticle diffusion on the yield of the liquid-phase hydrogenation of phenylacetylene in a stirred basket reactor.
- The effectiveness factor of a catalyst pellet in the liquid-phase hydrogenation of styrene.
- An empirical kinetic model and simulation for anodic oxidation of coal slurry.
- Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering
- Cesium accumulation of Rhodococcus erythropolis CS98 strain immobilized in hydrogel matrices(BIOCHEMICAL ENGINEERING)