Hepatic Differentiation of Mouse Embryonic Stem Cells in a Three-Dimensional Culture System Using Polyurethane Foam(CELL AND TISSUE ENGINEERING)

スポンサーリンク

概要

• 論文の詳細を見る

• Embryonic stem(ES) cells are a type of pluripotent stem cell line isolated from the inner cell mass of blastocysts and characterized by an almost unlimited self-renewal capacity and differentiation potential in vitro into multiple cell lineages. Therefore the use of ES cells has recently received much attention as a novel cell source for various hybrid artificial organs. To use ES cells, it is necessary to be able to produce functional matured cells from ES cells in large quantities. In this study, we applied polyurethane foam (PUF)/spheroid culture, which enables spontaneous spheroid formation and mass cultivation of cultured cells, to mouse ES cells for hepatic differentiation. Mouse ES cells spontaneously formed spherical multicellular aggregates(spheroids) in the pores of the PUF within 1 d. To induce hepatic differentiation, specific growth factors were added to the culture medium. Mouse ES cells proliferated by day 20, and high cell density(about 1.0×10^8cells/cm^3-PUF) was achieved. Differentiating ES cells expressed endodermal-specific genes, such as α-fetoprotein, albumin and tryptophan 2,3-dioxygenase. The activity of ammonia removal of mouse ES cells per unit volume of the module was detected by day 21 and increased with culture time. Maximum expression levels were comparable to those of primary mouse hepatocytes. Mouse ES cells could express liver-specific functions at high level because of the high cell density culture and hepatic differentiation. These results suggest that the PUF/spheroid culture method could be useful to develop mass differentiation cultures.

• 社団法人日本生物工学会の論文
• 2008-04-25

著者

• Nakazawa Kohji

  Department of Life and Environment Engineering, The University of Kitakyushu

• KAJIWARA Toshihisa

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Ijima Hiroyuki

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Nakazawa Kohji

  Department Of Chemical Processes And Environments The University Of Kitakyushu

• FUNATSU Kazumori

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Funatsu Kazumori

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Mizumoto Hiroshi

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Matsumoto Kinya

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Kajiwara Toshihisa

  Dep. Of Chemical Engineering Fac. Of Engineering Kyushu Univ.

• Kajiwara Toshihisa

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Ijima Hiroyuki

  Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University

• Matsumoto Kinya

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Mizumoto Hiroshi

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

関連論文

• Three-dimensional viscoelastic analysis of multi-layer polymer flow by numerical simulation (計算機レオロジー特集号)
• Alkoxyresorufin O-dealkylase assay using a rat hepatocyte spheroid microarray(CELL AND TISSUE ENGINEERING)
• Preparation of cell-enclosing microcapsules through photopolymerization of methacrylated alginate solution triggered by irradiation with visible light(BIOCHEMICAL ENGINEERING)
• Effect of microwell chip structure on cell microsphere production of various animal cells(CELL AND TISSUE ENGINEERING)
• Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes(CELL AND TISSUE ENGINEERING)
• Preparation of SiO2–PVA nanocomposite and monolithic transparent silica glass by sintering
• Development of a hybrid artificial liver support system and preclinical animal experiments
• Enhancement of Cell Function through Heterotypic Cell-Cell Interactions Using E-Cadherin-Expressing NIH3T3 Cells(CELL AND TISSUE ENGINEERING)
• The formation of a spheroid of primary hepatocytes and the expression of liver-specific functions depend on the characteristics of polyurethane foam
• Albumin production activity of primary rat hepatocytes is improved on type V collagen(CELL AND TISSUE ENGINEERING)
• Micropatterned Organoid Culture of Rat Hepatocytes and HepG2 Cells(CELL AND TISSUE ENGINEERING)
• Enhancement of the Activity of Renatured Lysozyme by Protein Disulfide Isomerase(BIOCHEMICAL ENGINEERING)
• Novel Technique for Fabricating Double-Layered Tubular Constructs Consisting of Two Vascular Cell Types in Collagen Gels Used as Templates for Three-Dimensional Tissues(CELL AND TISSUE ENGINEERING)
• Expression of a Liver-Specific Function by a Hepatoblastoma Cell Line Cocultured with Three-Dimensional Endothelialized Tubes in Collagen Gels(CELL AND TISSUE ENGINEERING)
• Enhanced Angiogenesis in bFGF-Containing Scaffold Promoted Viability of Enclosed Hepatocytes and Maintained Hepatospecific Glycogen Storage Capacity
• Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces
• Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes(CELL AND TISSUE ENGINEERING)
• Hepatic Differentiation of Mouse Embryonic Stem Cells in a Three-Dimensional Culture System Using Polyurethane Foam(CELL AND TISSUE ENGINEERING)
• Evaluation of instability after transtrochanteric anterior rotational osteotomy for nontraumatic osteonecrosis of the femoral head
• Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips(CELL AND TISSUE ENGINEERING)
• The Mixed Coculture Effect of Primary Rat Hepatocytes and Bone Marrow Cells Is Caused by Soluble Factors Derived from Bone Marrow Cells(CELL AND TISSUE ENGINEERING)
• Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science and Engineering)
• Promotion of Monolayer Formation and High Expression of Ammonia Metabolism of Primary Rat Hepatocytes on Arginine-Glycine-Aspartic Acid-Containing Peptide-Coated Polystyrene Dish(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science a
• Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period
• Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum : Use as an Animal Cell Culture Substratum
• Permeability of a sol-gel synthesized aminopropyl-silicate-titanate hybrid membrane for the microcapsule-shaped bioartificial pancreas
• Development of a Hybrid Artificial Liver Based on Hepatocyte Organoid Culture Technique for Chronic Liver Failure Patients
• Numerical Simulation of Extrudate Swell Problem and Evaluation of Applicability of Viscoelastic Constitutive Models : 1. A Study on Axisymmetric Extrudate Swell from a Straight Die
• Noncontact measurement of viscosity of B2O3 melt using electric-field tweezers (ナノ/マイクロレオロジー特集号)
• Afterglow luminance property of phosphorescent phosphor SrAl2O4:Eu2+,Dy3+-glass composites
• Reconstruction of Cartilage Tissue Using Scaffold-Free Organoid Culture Technique(CELL AND TISSUE ENGINEERING)
• Relationship between Neck-in Phenomena and Rheological Properties in Film Casting (工業レオロジー特集号)
• Large-Scale Cultivation of Embryonic and Other Stem Cells, Induced Differentiation into Various Cell Types, and Development of Bio-artificial Organ Systems
• Antimicrobial effect of porcelain glaze with silver-clay antimicrobial agent
• Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips
• Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis(CELL AND TISSUE ENGINEERING)
• In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications(CELL AND TISSUE ENGINEERING)
• Relationship between Neck-in Phenomena and Rheological Properties in Film Casting (工業レオロジー特集号)
• Noncontact measurement of viscosity of B2O3 melt using electric-field tweezers (ナノ/マイクロレオロジー特集号)
• Micropatterned Organoid Culture of Rat Hepatocytes and HepG2 Cells
• Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering(CELL AND TISSUE ENGINEERING)
• Heparin-conjugated gelatin as a growth factor immobilization scaffold(CELL AND TISSUE ENGINEERING)
• Nucleus number in clusters of transplanted fetal liver cells increases by partial hepatectomy of recipient rats(CELL AND TISSUE ENGINEERING)
• THE BEHAVIOURS OF HUMAN ENDOMETRIAL CELLS IN VARIOUS ENDOCRINOLOGICAL CONDITIONS
• Capturing the Efficiency of a Melt-Mixing Process for Polymer Processing
• Characterization of mouse embryoid bodies cultured on microwell chips with different well sizes(CELL AND TISSUE ENGINEERING)
• Solubilized matrix derived from decellularized liver as a growth factor-immobilizable scaffold for hepatocyte culture(CELL AND TISSUE ENGINEERING)
• Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering

もっと見る 閉じる

スポンサーリンク