Promotion of Monolayer Formation and High Expression of Ammonia Metabolism of Primary Rat Hepatocytes on Arginine-Glycine-Aspartic Acid-Containing Peptide-Coated Polystyrene Dish(CELL AND TISSUE ENGINEERING, <Special Issue>Advances in Biomedical Science a
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概要
- 論文の詳細を見る
Cell adhesive peptide Arg-Gly-Asp (RGD) was immobilized using ProNectin F (PnF) on a nontreated polystyrene petri dish at a PnF density of 20ng cm^<-2>, which is sufficient for primary rat hepatocyte immobilization. The density of PnF on the dish affects cell morphology and expression of the differentiated functions within the range of 2-2500ng cm^<-2>. An optimal monolayer state with defined cell boundaries and hepatocyte nuclei was formed on a 2500ng cm^<-2> PnF-coated petri dish, and the ammonia metabolic function was expressed at as high a level as in the hepatocyte/spheroid. We conclude that 2500ng cm^<-2> PnF enhances the morphological stability and expression of liver-specific functions of the hepatocyte.
- 社団法人日本生物工学会の論文
- 2005-07-25
著者
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Kawakami Koei
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Ijima Hiroyuki
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Ijima Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
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Kawakami Koei
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Ijima Hiroyuki
Dep. Of Chemical Engineering Fac. Of Engineering Graduate School Kyushu Univ. 744 Motooka Nishi-ku F
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Kawakami Koei
Department Of Chemical Engineering Graduate School Of Engineering Kyushu University
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