Enzymatic Hydrolysis of Soluble Starch in a Polyethylene Glycol-Dextran Aqueous Two-Phase System
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概要
- 論文の詳細を見る
Hydrolysis of soluble starch by glucoamylase and β-amylase was investigated as a model reaction in an aqueous two-phase system consisting of polyethylene glycol (PEG) and dextran (DEX). Changes in glycose concentration observed in the batch reaction experiments with glucoamylase were almost identical for the aqueous two-phase and pure water systems, showing that the enzymic reactions investigated were not influenced by the presence of PEG and DEX. The partition of β-amylase into the DEX phase was insufficient compared to that of glucoamylase. Hence, the former enzyme was crosslinked with glutaraldehyde to increase its apparent molecular weight and, as a consequence, the partition coefficient, defined as the concentration ratio of the component partitioned into the PEG phase to that into the DEX phase, was decreased to 17% of that of the original enzyme. In the operation in which the enzyme and substrate are partitioned selectively into the DEX phase and allowed to react there while the product, thus transferring to the PEG phase, is recovered, the aqueous two-phase system with a smaller partition coefficient provided longer operational stability.
- 公益社団法人日本生物工学会の論文
- 1990-04-25
著者
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Kawakami Koei
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Arai Yasuhiko
Department Of Chemical Engineering Kyushu University
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Shiraishi F
Kyushu Inst. Technol. Iizuka Jpn
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Shiraishi Fumihide
Department Of Biochemical Engineering And Science Faculty Of Computer Science Adn Systems Engineerin
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HAYASHIDA KAZUHIKO
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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KUNIMOTO KOJI
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Kunimoto Koji
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Hayashida K
Kyushu Univ. Fukuoka Jpn
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Hayashida Kazuhiko
Department Of Chemical Engineering Kyushu University
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Arai Yasuhiko
Department Of Chemical Engineering
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ARAI YASUHIKO
Department of Chemical Engineeering, Tohoku University
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