Synthesis of an Agarose-Gelatin Conjugate for Use as a Tissue Engineering Scaffold(CELL AND TISSUE ENGINEERING)

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概要

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• We synthesized a conjugate in which gelatin was covalently crosslinked to agarose using 1,1-carbonyldiimidazole (CDI) in dimethyl sulfoxide in order to obtain gels with cellular adhesiveness that showed a sol-to-gel transition, but no gel-to-sol transition, around body temperature. The gelatin content of the conjugate increased by 2.7-fold when the concentration of CDI was increased from 1.3 to 32.7mM. Aqueous solutions of the conjugate gelled upon cooling from 40℃ to 20℃, but did not remelt at 37℃. The percentage of adhered cells after 4h of culture on a gel created from a conjugate containing about 25wt% gelatin was similar to that for cells grown on tissue culture dishes. The adhered cells proliferated on the conjugate gel during culture for a further 5 d. In addition, the conjugate used in this study did not result in mechanical instability of the resultant gel compared to that of an unmodified agarose gel. These results demonstrate that this agarose-gelatin conjugate is a good candidate material for tissue engineering.

• 公益社団法人日本生物工学会の論文
• 2007-01-25

著者

• Sakai Shinji

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Sakai Shinji

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Kawakami Koei

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Hashimoto Ichiro

  Department Of Dermatology School Of Medicine The University Of Tokushima

• Hashimoto Ichiro

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Hashimoto Ichiro

  Department Of Biotechnology Tottori University

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