The Mixed Coculture Effect of Primary Rat Hepatocytes and Bone Marrow Cells Is Caused by Soluble Factors Derived from Bone Marrow Cells(CELL AND TISSUE ENGINEERING)

スポンサーリンク

概要

• 論文の詳細を見る

• Heterospheroids consisting of hepatocytes and bone marrow cells(BMCs) are formed by the mixed coculture of these cells and enhance the expression and maintenance of the liver-specific functions of hepatocytes. Not only the soluble factors derived from these cells, but also functional organoid(heterospheroid) formation, are considered to underlie this coculture effect. Therefore, in the present study, we aimed to clarify the mechanism of this co-culture effect. We performed hepatocyte monoculture with conditioned media prepared from hepatocyte cultures, BMC cultures and a coculture of hepatocytes and BMCs. When using any type of conditioned medium, no hepatocyte spheroids formed, and the hepatocytes formed a monolayer. In addition, an effect for these conditioned media was shown in terms of the albumin production and ammonia metabolism activities of the hepatocytes; conditioned medium from BMCs showed the strongest effect. The monocultured hepatocytes in the conditioned medium derived from BMCs showed equivalent albumin production and ammonia metabolism activities to the cocultured spheroids of hepatocytes and BMCs. Therefore, it was determined that the effect of the coculture of hepatocytes and BMCs was caused by soluble factors derived from BMCs.

• 社団法人日本生物工学会の論文
• 2008-03-25

著者

• Kawakami Koei

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Ijima Hiroyuki

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Matsuo Takeshi

  Department of Internal Medicine, Miyazaki Social Insurance Hospital

• Ijima Hiroyuki

  Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University

• Kawakami Koei

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Ijima Hiroyuki

  Dep. Of Chemical Engineering Fac. Of Engineering Graduate School Kyushu Univ. 744 Motooka Nishi-ku F

• Matsuo Takeshi

  Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University

• Kawakami Koei

  Department Of Chemical Engineering Graduate School Of Engineering Kyushu University

関連論文

• Heat treatment of electrospun silicate fiber substrates enhances cellular adhesion and proliferation(CELL AND TISSUE ENGINEERING)
• Preparation of cell-enclosing microcapsules through photopolymerization of methacrylated alginate solution triggered by irradiation with visible light(BIOCHEMICAL ENGINEERING)
• Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes(CELL AND TISSUE ENGINEERING)
• Heat treatment of electrospun silicate fiber substrates enhances cellular adhesion and proliferation
• PJ-042 Associations of Plasma Pentraxin 3, TNF α, and MCP-1 Concentrations with Cardiovascular Disease in Patients with Chronic Kidney Disease(PJ007,Kidney/Renal Circulation/CKD 1 (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Jap
• PJ-469 Down Regulation of Plasma Pentraxin 3 in Subjects with Metabolic Syndrome(Diabetes/Obesity/Metabolic syndrome(11)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• PE-219 Imbalance of Cholesterol Synthesis and Absorption in Excess Obesity(PE037,Obesity/SAS 1 (H),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
• Transesterification by Lipase Entrapped in Electrospun Poly(Vinyl Alcohol) Fibers and Its Application to a Flow-Through Reactor(BIOCHEMICAL ENGINEERING)
• Development of a hybrid artificial liver support system and preclinical animal experiments
• Enhancement of Cell Function through Heterotypic Cell-Cell Interactions Using E-Cadherin-Expressing NIH3T3 Cells(CELL AND TISSUE ENGINEERING)
• Albumin production activity of primary rat hepatocytes is improved on type V collagen(CELL AND TISSUE ENGINEERING)
• FRS-078 Cholesterol Synthesis and Absorption in Subjects with Metabolic Syndrome(FRS16,Basic Mechanisms of Lipid Disorders and Metabolic Syndrome (H),Featured Research Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Societ
• Enhancement of the Activity of Renatured Lysozyme by Protein Disulfide Isomerase(BIOCHEMICAL ENGINEERING)
• Novel Technique for Fabricating Double-Layered Tubular Constructs Consisting of Two Vascular Cell Types in Collagen Gels Used as Templates for Three-Dimensional Tissues(CELL AND TISSUE ENGINEERING)
• Expression of a Liver-Specific Function by a Hepatoblastoma Cell Line Cocultured with Three-Dimensional Endothelialized Tubes in Collagen Gels(CELL AND TISSUE ENGINEERING)
• Enhanced Angiogenesis in bFGF-Containing Scaffold Promoted Viability of Enclosed Hepatocytes and Maintained Hepatospecific Glycogen Storage Capacity
• Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces
• Activity and Activity Coefficient of Water in Aqueous Solutions and Their Relationships with Solution Structure Parameters
• Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes(CELL AND TISSUE ENGINEERING)
• Hepatic Differentiation of Mouse Embryonic Stem Cells in a Three-Dimensional Culture System Using Polyurethane Foam(CELL AND TISSUE ENGINEERING)
• Synthesis of Polycyclic Compounds by the Reaction of Co_2(CO)_6 Complexed 1-[o-(1-Alkynyl)phenyl]cyclopropanols
• The Mixed Coculture Effect of Primary Rat Hepatocytes and Bone Marrow Cells Is Caused by Soluble Factors Derived from Bone Marrow Cells(CELL AND TISSUE ENGINEERING)
• Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science and Engineering)
• Promotion of Monolayer Formation and High Expression of Ammonia Metabolism of Primary Rat Hepatocytes on Arginine-Glycine-Aspartic Acid-Containing Peptide-Coated Polystyrene Dish(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science a
• Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period
• Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum : Use as an Animal Cell Culture Substratum
• Permeability of a sol-gel synthesized aminopropyl-silicate-titanate hybrid membrane for the microcapsule-shaped bioartificial pancreas
• Microvascular Dysfunction in Patients with Coronary Vasospastic Angina
• CONTINUOUS PRODUCTION OF ACETIC ACID FROM CO_2 IN REPEATED-BATCH CULTURES USING FLOCCULATED CELLS OF ACETOBACTERIUM WOODII
• QUANTITATIVE ANALYSIS OF FLOCCULATION OF ACETOBACTERIUM WOODII BY CHEMICAL FLOCCULANTS
• Development of a Hybrid Artificial Liver Based on Hepatocyte Organoid Culture Technique for Chronic Liver Failure Patients
• USEFULLNESS OF CORONARY ANGIOPLASTY (PTCA) OF THE INFARCT-RELATED ARTERY IN THE PATIENTS WITH PRIOR MYOCARDIAL INFARCTION : RI : 53 Annual Scientific Meeting, Japanese Circulation Society
• -P51-CLINICAL APPLICATION OF AN AMBULATORY MONITORING SYSTEM (VEST) WITH CADMIUM TELLURIDE (CdTe) DETECTOR : EVALUATION OF LEFT VENTRICULAR FUNCTION IMMEDIATELY AFTER EXERCISE : RI : FREE COMMUNICATIONS(III) : PROCEEDINGS OF THE 53th ANNUAL SCIENTIFIC MEE
• Granular Cell Tumor of the Breast: A Case Report Describing Dynamic MR Mammography
• Thermal Stabilization of Lipase by Sol-Gel Entrapment in Organically Modified Silicates Formed on Kieselguhr
• Large-Scale Cultivation of Embryonic and Other Stem Cells, Induced Differentiation into Various Cell Types, and Development of Bio-artificial Organ Systems
• Synthesis of Enzymatically-Gellable Carboxymethylcellulose for Biomedical Applications(CELL AND TISSUE ENGINEERING)
• Synthesis of an Agarose-Gelatin Conjugate for Use as a Tissue Engineering Scaffold(CELL AND TISSUE ENGINEERING)
• Rearrangement of 1-Alkynylcyclopropanol to 3-Substituted 2-Cyclopentenone Promoted by a Catalytic Amount of Octacarbonyldicobalt-Triphenylphosphite
• Octacarbonyldicobalt Promoted Transformation of 1-(1,2-Propadienyl)cyclopropanols to 1,4-Hydroquinones
• Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis(CELL AND TISSUE ENGINEERING)
• In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications(CELL AND TISSUE ENGINEERING)
• Enzymatic Hydrolysis of Soluble Starch in a Polyethylene Glycol-Dextran Aqueous Two-Phase System
• Enhancement of Enzymatic Production of Cyclodextrins by Adding Polyethylene Glycol or Polypropylene Glycol
• Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering(CELL AND TISSUE ENGINEERING)
• Controlling the Diameters of Silica Nanofibers Obtained by Sol-Gel/Electrospinning Methods
• Controlling the Diameters of Silica Nanofibers Obtained by Sol–Gel/Electrospinning Methods
• Heparin-conjugated gelatin as a growth factor immobilization scaffold(CELL AND TISSUE ENGINEERING)
• Nucleus number in clusters of transplanted fetal liver cells increases by partial hepatectomy of recipient rats(CELL AND TISSUE ENGINEERING)
• The individual and competitive hydrogenations of ethylene and propylene in a stirred slurry reactor.
• Solubilized matrix derived from decellularized liver as a growth factor-immobilizable scaffold for hepatocyte culture(CELL AND TISSUE ENGINEERING)
• The effects of intraparticle diffusion on the yield of the liquid-phase hydrogenation of phenylacetylene in a stirred basket reactor.
• The effectiveness factor of a catalyst pellet in the liquid-phase hydrogenation of styrene.
• An empirical kinetic model and simulation for anodic oxidation of coal slurry.
• Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering

もっと見る 閉じる

スポンサーリンク