Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes(CELL AND TISSUE ENGINEERING)
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概要
- 論文の詳細を見る
Hepatocyte organoids have an in vivo-like cell morphology and maintain cell viability and function in vitro. On the other hand, the oxygen supply to hepatocytes is sometimes limited in the core of organoids that are more than 100μm in thickness. In this study, we designed and examined a new bioreactor using sheet-shaped organoids (organoid-sheets) in which the thickness was controlled to prevent hepatocyte death in the core of organoid due to limitation of oxygen supply. The cell culture space consisted of stacked organoid formation spaces and medium flow channels. Each space was separated by flat porous polycarbonate membranes, and the organoid thickness was controlled at 100μm with a stainless steel spacer. Freshly isolated hepatocytes (7.0×10^7) were immobilized in the bioreactor, yielding a cell density of 4.5×10^7cells/cm^3-bioreactor. Of the five flow rates tested (1.0, 5.0, 10, 20, and 50mL/min), the bioreactor with the 10mL/min had the highest ammonia removal and albumin secretion activities for at least 14 days. In conclusion, a new bioreactor controlling organoid thickness is useful for achieving high cell density culture and the maintenance of hepatocyte function to avoid cell death in the core of the organoids due to limitation of oxygen supply. The bioreactor may be useful for the development of various applications using cultured hepatocytes.
- 社団法人日本生物工学会の論文
著者
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Nakazawa Kohji
Department of Life and Environment Engineering, The University of Kitakyushu
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KAJIWARA Toshihisa
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Ijima Hiroyuki
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Nakazawa Kohji
Department Of Chemical Processes And Environments The University Of Kitakyushu
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FUNATSU Kazumori
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Funatsu Kazumori
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Kusumi Tomoaki
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Ishihara Kazuhisa
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Mizumoto Hiroshi
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Ishihara Kazuhisa
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Kajiwara Toshihisa
Dep. Of Chemical Engineering Fac. Of Engineering Kyushu Univ.
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Kajiwara Toshihisa
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Ijima Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
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Kusumi Tomoaki
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Mizumoto Hiroshi
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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