Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum : Use as an Animal Cell Culture Substratum
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概要
- 論文の詳細を見る
A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150–200 μm, and a porosity of 28% that was dependent on the PMMA beads quolity. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·ml–1-HAp. This value is similar to most existing suitable porous microcarriers.A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.
- 社団法人 化学工学会の論文
- 2004-04-01
著者
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Kawakami Koei
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Ijima Hiroyuki
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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ONO Tsutomu
Department of Chemical Engineering, Faculty of Engineering, Kyushu University
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Ijima Hiroyuki
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
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Ono Tsutomu
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Kawakami Koei
Department Of Chemical Engineering Faculty Of Engineering Kyushu University
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Ijima Hiroyuki
Dep. Of Chemical Engineering Fac. Of Engineering Graduate School Kyushu Univ. 744 Motooka Nishi-ku F
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NAKASHIMA Motonori
Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University
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FURUTA Sachiko
Saga Ceramics Research Laboratory
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Nakashima Motonori
Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University
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Kawakami Koei
Department Of Chemical Engineering Graduate School Of Engineering Kyushu University
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