Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum : Use as an Animal Cell Culture Substratum

スポンサーリンク

概要

• 論文の詳細を見る

• A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150–200 μm, and a porosity of 28% that was dependent on the PMMA beads quolity. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·ml–1-HAp. This value is similar to most existing suitable porous microcarriers.A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

• 社団法人 化学工学会の論文
• 2004-04-01

著者

• Kawakami Koei

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Ijima Hiroyuki

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• ONO Tsutomu

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Ijima Hiroyuki

  Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University

• Ono Tsutomu

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Kawakami Koei

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Ijima Hiroyuki

  Dep. Of Chemical Engineering Fac. Of Engineering Graduate School Kyushu Univ. 744 Motooka Nishi-ku F

• NAKASHIMA Motonori

  Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University

• FURUTA Sachiko

  Saga Ceramics Research Laboratory

• Nakashima Motonori

  Department Of Chemical Engineering Faculty Of Engineering Graduate School Kyushu University

• Kawakami Koei

  Department Of Chemical Engineering Graduate School Of Engineering Kyushu University

関連論文

• Heat treatment of electrospun silicate fiber substrates enhances cellular adhesion and proliferation(CELL AND TISSUE ENGINEERING)
• Preparation of cell-enclosing microcapsules through photopolymerization of methacrylated alginate solution triggered by irradiation with visible light(BIOCHEMICAL ENGINEERING)
• Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes(CELL AND TISSUE ENGINEERING)
• Heat treatment of electrospun silicate fiber substrates enhances cellular adhesion and proliferation
• Transesterification by Lipase Entrapped in Electrospun Poly(Vinyl Alcohol) Fibers and Its Application to a Flow-Through Reactor(BIOCHEMICAL ENGINEERING)
• Development of a hybrid artificial liver support system and preclinical animal experiments
• Enhancement of Cell Function through Heterotypic Cell-Cell Interactions Using E-Cadherin-Expressing NIH3T3 Cells(CELL AND TISSUE ENGINEERING)
• Albumin production activity of primary rat hepatocytes is improved on type V collagen(CELL AND TISSUE ENGINEERING)
• Enhancement of the Activity of Renatured Lysozyme by Protein Disulfide Isomerase(BIOCHEMICAL ENGINEERING)
• Novel Technique for Fabricating Double-Layered Tubular Constructs Consisting of Two Vascular Cell Types in Collagen Gels Used as Templates for Three-Dimensional Tissues(CELL AND TISSUE ENGINEERING)
• Expression of a Liver-Specific Function by a Hepatoblastoma Cell Line Cocultured with Three-Dimensional Endothelialized Tubes in Collagen Gels(CELL AND TISSUE ENGINEERING)
• Enhanced Angiogenesis in bFGF-Containing Scaffold Promoted Viability of Enclosed Hepatocytes and Maintained Hepatospecific Glycogen Storage Capacity
• Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces
• Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes(CELL AND TISSUE ENGINEERING)
• Hepatic Differentiation of Mouse Embryonic Stem Cells in a Three-Dimensional Culture System Using Polyurethane Foam(CELL AND TISSUE ENGINEERING)
• NOVEL IMMOBILIZED ENZYMES UTILIZING REVERSED MICELLAR SOLUTIONS AS AN OPTICAL RESOLUTION TOOL
• The Mixed Coculture Effect of Primary Rat Hepatocytes and Bone Marrow Cells Is Caused by Soluble Factors Derived from Bone Marrow Cells(CELL AND TISSUE ENGINEERING)
• Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science and Engineering)
• Promotion of Monolayer Formation and High Expression of Ammonia Metabolism of Primary Rat Hepatocytes on Arginine-Glycine-Aspartic Acid-Containing Peptide-Coated Polystyrene Dish(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science a
• Transition of Mechanical Property of Porous Alginate Scaffold with Cells during Culture Period
• Hydrothermal Synthesis of Porous Hydroxyapatite Plates from Gypsum : Use as an Animal Cell Culture Substratum
• Permeability of a sol-gel synthesized aminopropyl-silicate-titanate hybrid membrane for the microcapsule-shaped bioartificial pancreas
• Development of a Hybrid Artificial Liver Based on Hepatocyte Organoid Culture Technique for Chronic Liver Failure Patients
• Extraction of Ethyl Ester of Polyunsaturated Fatty Acids by Utilizing Slug Flow Prepared by Microreactor
• SUBCELLULAR LOCALIZATION OF INNEXIN, GAP JUNCTION RELATED PROTEIN IN INSECT CELLS(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• IMMUNOCYTOCHEMICAL DEMONSTRATION OF GAP JUNCTION RELATED PROTEINS (INNEXIN) IN INSECT CULTURED CELLS(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• DNA Extraction by Cationic Reverse Micelles
• Thermal Stabilization of Lipase by Sol-Gel Entrapment in Organically Modified Silicates Formed on Kieselguhr
• Large-Scale Cultivation of Embryonic and Other Stem Cells, Induced Differentiation into Various Cell Types, and Development of Bio-artificial Organ Systems
• Extraction of DNA by Reversed Micelles
• Synthesis of Enzymatically-Gellable Carboxymethylcellulose for Biomedical Applications(CELL AND TISSUE ENGINEERING)
• Synthesis of an Agarose-Gelatin Conjugate for Use as a Tissue Engineering Scaffold(CELL AND TISSUE ENGINEERING)
• Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis(CELL AND TISSUE ENGINEERING)
• In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications(CELL AND TISSUE ENGINEERING)
• Enzymatic Hydrolysis of Soluble Starch in a Polyethylene Glycol-Dextran Aqueous Two-Phase System
• Enhancement of Enzymatic Production of Cyclodextrins by Adding Polyethylene Glycol or Polypropylene Glycol
• Formulation and Evaluation of Paclitaxel-Loaded Polymeric Nanoparticles Composed of Polyethylene Glycol and Polylactic Acid Block Copolymer
• Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering(CELL AND TISSUE ENGINEERING)
• Controlling the Diameters of Silica Nanofibers Obtained by Sol-Gel/Electrospinning Methods
• Controlling the Diameters of Silica Nanofibers Obtained by Sol–Gel/Electrospinning Methods
• Heparin-conjugated gelatin as a growth factor immobilization scaffold(CELL AND TISSUE ENGINEERING)
• Nucleus number in clusters of transplanted fetal liver cells increases by partial hepatectomy of recipient rats(CELL AND TISSUE ENGINEERING)
• The individual and competitive hydrogenations of ethylene and propylene in a stirred slurry reactor.
• Solubilized matrix derived from decellularized liver as a growth factor-immobilizable scaffold for hepatocyte culture(CELL AND TISSUE ENGINEERING)
• The effects of intraparticle diffusion on the yield of the liquid-phase hydrogenation of phenylacetylene in a stirred basket reactor.
• The effectiveness factor of a catalyst pellet in the liquid-phase hydrogenation of styrene.
• An empirical kinetic model and simulation for anodic oxidation of coal slurry.
• Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering

もっと見る 閉じる

スポンサーリンク