Synthesis of Enzymatically-Gellable Carboxymethylcellulose for Biomedical Applications(CELL AND TISSUE ENGINEERING)

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概要

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• We synthesized a carboxymethylcellulose with phenol moieties by covalently incorporating tyramine into carboxymethylcellulose using aqueous-phase carbodiimide activation chemistry. The resulting hydrogel was obtained from an aqueous solution of the conjugate via the horseradish peroxidase-catalyzed oxidation reaction of phenols by consuming H_2O_2, where the gelation speed depended on the concentrations of enzyme and H_2O_2. The viability of the mammalian cells enclosed within the hydrogel prepared from 1.5%(w/v) conjugate solution containing 5 units/ml horseradish peroxidase and 1 mM H_2O_2, was 80% after 24 h. These results demonstrate that this carboxymethylcellulose with phenol moieties has potential for biomedical applications including tissue-engineering.

• 公益社団法人日本生物工学会の論文
• 2007-07-25

著者

• Sakai Shinji

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Kawakami Koei

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

• Ogushi Yuko

  Department of Chemical Engineering, Faculty of Engineering, Kyushu University

• Ogushi Yuko

  Department Of Chemical Engineering Faculty Of Engineering Kyushu University

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