Diffusional and Electrostatic Effects on Apparent Kinetic Parameters of Reactions Catalyzed by Enzyme Immobilized on the External Surface of a Support
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概要
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Diffusional and electrostatic effects on the apparent maximum reaction rate V_m^<app> and the apparent Michaelis constant K_m^<app> were investigated theoretically for a system in which an enzyme immobilized on the external surface of a solid support catalyzes a reaction according to Michaelis-Menten kinetics. In such a system, the dependence of V_m^<app> and K_m^<app> on the substrate concentration can be expressed analytically. When the support and substrate carry charges of the same sign, resulting in a repulsive force between them, both V_m^<app> and K_m^<app> decrease with increasing substrate concentration, but they never decrease below the respective intrinsic values. On the other hand, when the support and substrate carry charges of opposite sign and therefore an attractive force occurs, V_m^<app> decreases towards its intrinsic value, while K_m^<app> decreases to values below its intrinsic value in the region of high substrate concentration.
- 公益社団法人日本生物工学会の論文
- 1995-04-25
著者
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Shiraishi Fumihide
Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin
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Shiraishi Fumihide
Department Of Biochemical Engineering And Science Faculty Of Computer Science Adn Systems Engineerin
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