Characterization of Lactobacillus spp. isolated from the feces of breast-feeding piglets(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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Lactobacillus spp., referred to as IJ-1 and IJ-2, were isolated from the feces of breast-feeding piglets and analyzed for probiotic properties. According to the analyses of 16S rDNA sequence, Lactobacillus sp. IJ-1 showed greater than 99% homology with Lactobacillus reuteri DSM20016^T, and Lactobacillus sp. IJ-2 had greater than 99% homology with the L. gasseri ATCC33323^T and L. johnsonii ATCC33200^T. The pH changes in the culture media of Lactobacillus sp. IJ-1 and Lactobacillus sp. IJ-2 were from 6.5 to 4.2 and 4.6, respectively. Their respective resistance against artificial gastric acid and artificial bile acid led to survival rates of nearly 186±44% und 13±5%. Neither strain produced the carcinogenic enzyme β-glucuronidase. Both strains inhibited the growth of pathogenic microorganisms, such as Listeria monocytogenes ATCC19111, Salmonella enterica KCTC12401, Salmonella enteritidis ATCC13076, Staphylococcus aureus KCTC3881, and Bacillus cereus 3711, within 24h of growth.
社団法人日本生物工学会の論文