Actinomycin D Identified as an Inhibitor of Discoidin Domain Receptor 2 Interaction with Collagen through an Insect Cell Based Screening of a Drug Compound Library(Pharmacology)
スポンサーリンク
概要
- 論文の詳細を見る
Discoidin domain receptors belong to the cell surface receptor tyrosine kinase family and recognize collagens for their activating ligands. They have been implicated for cell growth and migration and their elevated expressions were observed in various human cancers. When we expressed human Discoidin domain receptor 2 (DDR2) in insect cells, the protein was targeted properly into the cell membrane and this could enforce the cells to adhere on culture plate coated with type I collagen. By taking advantage of this, we established a novel insect cell based screening protocol to identify chemicals which inhibit the interaction between DDR2 and collagen. We screened a drug-compound library to select an anti-cancer drug, actinomycin D, as the inhibitory compound. Actinomycin D prevented the activation of DDR2 by type I collagen in human embryonic kidney 293 cells with an IC_<50> value of 9μm, while it did not interfere with the activation of other receptor tyrosine kinases by their ligands. In conclusion we identified a new biological function of actinomycin D and the insect cell based method provides a useful protocol for screening inhibitors against the association of DDR2 with collagen.
- 2009-01-01
著者
-
SIDDIQUI Kauser
Biomedical Research Center, Korea Institute of Science and Technology
-
KIM Geun
Biomedical Research Center, Korea Institute of Science and Technology
-
LEE Dong
Biomedical Research Center, Korea Institute of Science and Technology
-
SHIN Hae
Biomedical Research Center, Korea Institute of Science and Technology
-
YANG Eun
Biomedical Research Center, Korea Institute of Science and Technology
-
LEE Nam
Department of Chemistry, Korea Military Academy
-
YANG Beom-Seok
Biomedical Research Center, Korea Institute of Science and Technology
-
Lee Nam
Department Of Biochemistry Dongguk University
-
Kim Geun
Biomedical Research Center Korea Institute Of Science And Technology
-
Yang Eun
Biomedical Research Center Korea Institute Of Science And Technology
-
Shin Hae
Biomedical Research Center Korea Institute Of Science And Technology
-
Yang Beom-seok
Biomedical Research Center Korea Institute Of Science And Technology
-
Lee Dong
Dep. Of Environmental And Health Chemistry Coll. Of Pharmacy Chung-ang Univ.
-
Siddiqui Kauser
Biomedical Research Center Korea Institute Of Science And Technology
-
Lee Nam
Department Of Chemistry Korea Military Academy
-
Lee Dong
Biomedical Research Center Korea Institute Of Science And Technology
関連論文
- Actinomycin D Identified as an Inhibitor of Discoidin Domain Receptor 2 Interaction with Collagen through an Insect Cell Based Screening of a Drug Compound Library(Pharmacology)
- 1,2,3,4,6-Penta-O-galloyl-β-D-glucose Protects Splenocytes against Radiation-Induced Apoptosis in Murine Splenocytes
- Isolation and characterization of a novel analyte from Bacillus subtilis SC-8 antagonistic to Bacillus cereus(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Antimutagenicity and Cytotoxicity of the Constituents from the Aerial Parts of Rumex acetosa(Pharmacognosy)
- Protective Effect of Triphlorethol-A from Ecklonia cava against Ionizing Radiation in vitro
- IS-020 Prenatal Diagnostic Clues for Fetus-in-fetu(the 44th Annual Meeting of Japanese Society of Pediatric Surgeons)
- Olfactory Brain Evoked Potentials to Odorant Stimulations : Effects of Age on Smell Sensitivity
- Antimutagenic Activity of 5α-Cholest-7-en-3β-ol, a New Component from the Starfish Asterina pectinifera
- Characterization of Lactobacillus spp. isolated from the feces of breast-feeding piglets(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effect of Palmitoleic Acid on Melanogenic Protein Expression in Murine B16 Melanoma
- A Case of Pre-Sezary Syndrome Preceded by Hand Lesions
- The Glycosylation and in Vivo Stability of Human Granulocyte-Macrophage Colony-Stimulating Factor Produced in Rice Cells(Biopharmacy)
- Poisson Equations with Nonlinear Source Terms on Networks
- Depigmentation of Melanocytes by (2Z,8Z)-Matricaria Acid Methyl Ester Isolated from Erigeron breviscapus
- Tepidimonas arfidensis Sp. Nov., a Novel Gram-Negative and Thermophilic Bacterium Isolated from the Bone Marrow of a Patient with Leukemia in Korea
- Biological Activities of Korean Citrus obovoides and Citrus natsudaidai Essential Oils against Acne-Inducing Bacteria
- Chemical Composition and Anti-inflammatory Effects of Essential Oil from Farfugium japonicum Flower
- Sasa quelpaertensis Phenylpropanoid Derivative Suppresses Lipopolysaccharide-induced Nitric Oxide Synthase and Cyclo-oxygenase-2 Expressions in RAW 264.7 Cells
- Biological Activities of Korean Citrus obovoides and Citrus natsudaidai Essential Oils against Acne-Inducing Bacteria
- Electrical Properties of Self-Assembled Monolayers Using STM
- Prenatal diagnostic clue for fetus in fetu
- Whole-Blood Interferon-Gamma Release Assay for Diagnosis of Tuberculous Lymphadenitis
- Narrow antagonistic activity of antimicrobial peptide from Bacillus subtilis SCK-2 against Bacillus cereus(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Mycobacterium abscessus Pulmonary Infection Presenting as a Solitary Pulmonary Nodule
- Acute meningitis as an initial manifestation of Erysipelothrix rhusiopathiae endocarditis
- Direct-Puncture Embolization of Scalp Arteriovenous Fistulae : Technical Note on the Circular Compression Device
- Phloroglucinol Exerts Protective Effects Against Oxidative Stress-Induced Cell Damage in SH-SY5Y Cells
- Phloroglucinol Exerts Protective Effects Against Oxidative Stress–Induced Cell Damage in SH-SY5Y Cells
- Eckol Inhibits Ultraviolet B-Induced Cell Damage in Human Keratinocytes via a Decrease in Oxidative Stress
- Actinomycin D Identified as an Inhibitor of Discoidin Domain Receptor 2 Interaction with Collagen through an Insect Cell Based Screening of a Drug Compound Library
- Effects of Clioquinol Analogues on the Hypoxia-Inducible Factor Pathway and Intracelullar Mobilization of Metal Ions
- Biosystematic Studies on the Family Tofieldiaceae IV. Taxonomy of Tofieldia coccinea in Japan and Korea Including a New Variety
- Photoprotective effect of Undaria crenata against ultraviolet B-induced damage to keratinocytes(MISCELLANEOUS)
- Electrostatic Force Microscopy of Metallic Ion-Intercalated DNA