Escherichia coli recA変異株の凍結乾燥耐性におよぼす培養温度のシフトアップの効果(昭和62年度第33回凍結及び乾燥研究会研究報告)

概要

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Every recA-deficient mutant was sensitive to freeze-drying. A shift-up of growth temperature from 30℃ to 42℃ during the logarithmic growth phase increased the viability after freeze-drying. The growth at the higher temperature (less than 42℃) gave the more tolerance to freeze-drying. Growth in the presence of cerulenin (20μg/ml) also increased viability after freeze-drying. When the mutants were grown at 42℃ with cerulenine and freeze-dried with thiourea, Na-glutamate and sucrose, much higher viability was observed after the freeze-drying. Some weak stresses on the cells, such as UV-irradiation (20μJ/cm^2) or treatment with 0.1% H_2O_2 for 3 min also increased the viability after freeze-drying. These methods might be useful to protect recA^+ strains from mutation through the freeze-drying.
低温生物工学会の論文
1987-09-30