4. 酵母細胞の凍結と融解の上でディメチル・スルフォキシド(DMSO)の影響(昭和58年度第29回凍結及び乾燥研究会特別講演及び研究報告)

概要

論文の詳細を見る
Cellular viability of various yeast strain were examined after freezing and thawing with or without cryoprotectant at -70℃. Sensitivity to the freeze-thawing, treatment was varied depending on the strains was modified by age of culture, growth media, protective solution and freezing conditions. Twenty-four hr culture was optimal for preservation by freezing. The optimum freezing rate depended on kind of protective agent, although protective effect of 10% dimethyl sulfoxide was observed at all of freezing rate and gave the highest survival among the agent tested. Polyvinylpyrrolidone failed to protect yeast cell subjected to freeze-thaw. Warming rate during thawing did not affect the viability of various Saccharomyces yeasts and unexpectedly high recoveries of them were obtained without cryoprotectant. Hodling period at an intermediate temperature was not necessary when cells were frozen at an optimum freezing rate. Some strains were very sensitive to the freezing-thawing process. However, in general more than 55% of survival rate was found when 10% dimethyl sulfoxide was used as a agent. Some of the yeast strains tested showed more than 90% of survival rate.
低温生物工学会の論文
1983-08-25