フェノール廃液から酵母によるカテコールの生産 : フェノール類の微生物による分析に関する研究(第7報)

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In order to produce catechol from phenol in industrial wastes, an isolated yeast was selected at the best producer of catechol from phenol, from among a few strains of phenol metabolizing yeasts. The isolate was examined with respect to its taxonomic characteristics and identified as Rhodotorula glutinis (Fres.) Harison var. glutinis Phaff et Ahearn.Using the cells of R. glutinis var. glutinis, precultured on glucose, optimum conditions for catechol formation were 10 mg/ml of cell concentration, 200 ml of medium volume in a 500 ml shaking flask, 300 mg/l of phenol concentration, and a temperature of 30℃. Without the inhibitor, 245 mg/l of catechol formed in 3 hr, at pH 1.5,and with 1 mM of α, α'-dipyridyl, 350 mg/l of catechol formed in 4 hr, at pH 4.0. In a jar fermenter, 2,000 mg/l of phenol fed successively was hydroxylated efficiently to 2,300 mg/l of catechol in 8 hr by R. glutinis var. glutinis.
1974-01-25