49(P-15) 果実およびバラの花におけるC_<13>-ノルイソプレノイド香気成分の生成機構(ポスター発表の部)

概要

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β-Damascenone (1) and the related C_<13>-norisoprenoid aroma compounds have been suggested to be produced through biodegradation or autooxidation of carotenoids in several fruits and rose flowers, although neither the enzymes nor the direct progenitors were clarified. We investigated the enzymatic cleavage of β-carotene in plant tissues, fruit and flowers. Fruit skin of quince (Cyndonia oblanga) and starfruit (Averrhoa carambola), and rose flowers (Rosa damascena Mill.) showed the enzymatic activities toward β-carotene. Kinetical and physical properties, such as MW, pI, K_M of fuit carotenases were clarified. This is the first instance for the detection of enzymatic activities, being involved in aroma formation, from plant tissues. We were able to detect several progenitors of 1 in flowers of R. damascena Mill. Guided by the detection of 1 and 3-hydroxy-bdamascone (3) by GC-MS, the progenitor fractions were purified to isolate one of the progenitors and the chemical structure was elucidated to be 3-hydroxy-7, 8-didehydro-β-ionol 9-O-β-D-glucopyranoside (4c). The rests were suggested to be allenic triol (2a) and its glucoconjugats (2b〜2d). Chracterization and the synthesis of the progenitors will be discribed.
天然有機化合物討論会の論文
2000-10-01