6 Diels-Alder反応を触媒する新規酵素、ソラナピロン合成酵素の性質および基質特異性(口頭発表の部)

概要

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Solanapyrone synthase has been partially purified from a phytopathogenic fungus Alternaria solani. On the basis of the chromatographic behavior, it is speculated that single enzyme catalyzes both oxidation of 6 and the subsequent Diels-Alder reaction of 7. The enzyme is monomeric and showed native molecular weight 40 kD and pl 4.25. The substrate specificity of this enzyme was also examined with various substrate analogs. Enzymatic Diels-Alder reaction of 6 in semi-preparative scale afforded (-)-1 with high enantioselectivity and with good exo-selectivity which is difficult to attain by chemical methods. In addition, the crude enzyme from A. solani has been able to perform a kinetic resolution of (±)-3.
天然有機化合物討論会の論文
1998-08-31