31 新規炭素炭素結合反応を触媒する酵素、マクロフォミン酸合成酵素の性質および反応機構(口頭発表の部)

概要

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Macrophomate synthase which catalyzes unusual multiple reactions from 2-pyrone (3) to macrophomic acid (1) has been purified as homogenous state. The macrophomate synthase is a dimeric enzyme which requires Mg^<2+> as a co-factor and whose molecular mass is 40kDa. Study on substrate specificity showed that the enzyme is capable of converting various 2-pyrones to the corresponding benzoates. The substrate specificity of this enzyme was also examined with various substrate analogs. Formation of an aberrant product 13 in the enzymatic reaction with 2-pyrone 11 strongly indicated involvement of a bicyclic intermediate which is readily degraded into aromatic product by a concerted dehydration-decarboxylation.
天然有機化合物討論会の論文
1999-09-01