88 脂肪酸生合成反応の立体化学 : 鎖の奇数番目の炭素上の水素について

概要

論文の詳細を見る
Incorporation of ^2H into fatty acid from NADPH and NADH, whose C_<4R or 4S>-position of hydropyridine ring was labeled with ^2H, was investigated utilizing fatty acid synthetases from rat liver, yeast, seed of Ricinus communis, Chlorella vulgaris, Cephalosporium caerulens, Brevibacterium ammoniagenes, Bacillus subtilis and Escherichia coli. GC-MS analyses of the methyl esters derived from the enzyme products showed that ^2H from the labeled reduced pyridine nucletides was incorporated on the odd numbered carbons of the fatty acid chain. To determine the stereospecificity of C_<4R or 4S>-^2H of NADH or NADPH, which is used in reduction of β-ketoacyl or enoyl group, the reactions of fatty acid synthetases with model substrates, such as S-acetoacetyl-N-acetylcysteamine or S-hydroxybutyryl-N-acetylcysteamine, were carried out under existence of ^2H-labeled reduced pyridine nucleotide. GC-MS analyses of the products clarified the stereospecificity in these reduction processes and the results were summarized in Table 1. As an approach for determining the absolute configuration of ^2H on the odd numbered carbon of fatty acid, which is incorporated from ^2H-labeled NADPH or NADH, stereochemistry of the reaction of 'key enzyme', acyl-CoA oxidase from peroxisome of rat liver, was studied.
天然有機化合物討論会の論文
1979-09-20