Unexpected Fragmentation of a Novel Cationic Glycosphingolipid, Glyceroplasmalopsychosine, by Electrospray Ionization and Low Energy Multistage Mass Spectrometry

概要

論文の詳細を見る
Glyceroplasmalopsychosine, Gro1(3)-O-plasmal-O-6Galβ-sphingosine, is a unique plasmal conjugate of galactosylsphingosine (psychosine) that has an O-acetal conjugate of plasmal to the hydroxyl groups of both glycerol and galactosyl residues. Electrospray ionization ion trap mass spectrometry was applied to the structural analysis of this unique compound. In the MS/MS (MS2) spectrum produced by low energy collision-induced dissociation (CID) of the [M+H]+ ion, elimination of glycerol was predominant. MS/MS/MS (MS3) of the [M+H-glycerol]+ ion yielded an outstanding anomalous ion which required the loss of a hexose in the middle of the molecule with the plasmal residue retained on the psyhchosine side. This unexpected fragmentation by MS3 could be attributed to a rearrangement of the plasmal residue to the sphingosine residue, allowing elimination of the galactose by subsequent MS3 after CID.
日本質量分析学会の論文
2002-04-01