The Major Acidic Glycolipids from the Kidney of the Pacific Salmon (Oncorhynchus keta): Characterization of a Novel Ganglioside, Fucosyl-N-acetylgalactosaminyl-GM1.

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Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, were eluted from a DEAE-Sephadex column in the monosialosyl fraction. The faster moving ganglioside (XI) on TLC was separated from the slower moving one (X 2) by HPLC using a silica beads column. By methylation analysis, chemical and enzymatic degradation, reaction with monoclonal antibodies, LSIMS, and 1H-NMR spectroscopy, X 1 was determined to be a monosialosyl ganglioside belonging to the ganglio-series with a unique Fucα1-3 GalNAc linkage at the nonreducing terminal: Fucal-3 Gal NAcβ1-3 Galβ1-3 GalNAcβ1-4 [NeuAcα2-3] Galβ1-4 Glcβ1-1 Cer Analysis of the lipophilic moiety indicated predominance of 24:1 fatty acid in combination with sphingenine. X 2 was found to have a glycon structure identical to X 1. The ceramide of X 2 consisted predominantly of saturated fatty acids (18:0 and 16:0). The tissue concentrations of X 1 and X 2 in kidney were 3.7 and 2.8 nmol/g, respectively.
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