Isolation and Characterization of a Novel Forssman Active Acidic Glycosphingolipid with Branched Isoglobo-, Ganglio-, and Neolacto-Series Hybrid Sugar Chains.

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概要

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• Equine kidney and spleen contain a Forssman active glycosphingolipid, and the structure of this glycolipid has been reported to be that of a globopentaosylceramide (GalNAcα-1, 3GalNAcβ-1, 3Galα-1, 4Galβ-1, 4Glcβ-1, 1'Ceramide). We found that equine kidney contains several other anti-Forssman antibody-reactive glycosphingolipids. One of these acidic Forssman active glycosphingolipids was isolated and characterized by means of NMR, mass spectrometry, permethylation studies, and TLC-immunostaining. This glycolipid contains three moles of galactose, one mole of glucose, three moles of N-acetylgalactosamine, one mole of N-acetylglucosamine, and one mole of N-acetylneuraminic acid, and is stained on TLC with anti-Forssman antibodies and anti-GM2 ganglioside antibodies. HOHAHA and ROESY experiments and permethylation studies showed this glycolipid oligosaccharide to be branched at the innermost galactose; one chain has an isoglobo structure with a terminal Forssman disaccharide and the other chain is branched through the linkage of N-acetylglucosamineβ-1, 6 to the inner galactose. The nonreducing end of the GM2 trisaccharide is linked to this glucosamine. The structure of the oligosaccharide of the glycolipid presented here is a novel type, having branched isoglobo-, ganglio-, and neolacto-series oligosaccharides. Mass spectrometric analyses indicated the ceramide moiety of the glycolipid to be composed predominantly of hydroxy fatty acids (C20:0, C22:0, C23:0, C24:0, and C25:0) and hydroxysphinganine. NeuAcα2 3 GaINAcβ-1, 4Galβ-1, 4GlcNAcβ-1, 6 Galβ-1, 4Glcβ-1, 1'Ceramide GalNAcα-1, 3GalNAcβ-1, 3Galα-1, 3

• 社団法人 日本生化学会の論文

著者

• Handa Shizuo

  Department Of Biochemistry Faculty Of Medicine Tokyo Medical And Dental University

• Ishizuka Ineo

  Department Of Biochemistry Teikyo University Medical School

• Yamamoto Harumi

  Department Of Biochemistry Faculty Of Medicine Tokyo Medical And Dental University

• Kasama Takeshi

  Department of Materials Science and Metallurgy, University of Cambridge, Cambridge CB2 3QZ, U.K.

• KUSHI Yasunori

  Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine

• Iida-Tanaka Naoko

  Instrumental Analysis Research Center for Life Science, Tokyo Medical and Dental University

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