327 プラスチック製DNAチップの高性能化の基礎研究(OS-16 微細加工と表面機能)

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The DNA chip is required of high sensitivity and low cost for practical medical service. We have tried to use polycarbonate (PC) included TiO_2 as a substrate of DNA chip for low cost. However, PC included TiO_2 cannot adhere DNA easily due to their hydrophobicity. In this work, in order to change the PC included TiO_2 to be hydrophilicity, Ar, N_2 and O_2 ions were irradiated on the surface of specimen by using an Electron Cyclotron Resonance (ECR) ion shower apparatus, respectively. We measured the contact angle of water on the surface of specimens with optical microscope. Surface morphology of specimens was observed by SEM, and measured by AFM. Surface of specimen analyzed by X-Ray photoelectron spectrometry (XPS). Then we measured adhesion force of DNA probe to the surface of specimen by force curve measurement using AFM in water. The contact angle of untreated specimen were decreased and reached to 8 deg. by N_2 and O_2 ion irradiation. The surface roughness of untreated specimen were increased and reached to 248nm by N_2 ion irradiation. The hydrophilic surface of specimen would be kept 1440 hours in air by 2 times irradiation of O_2 ion. XPS showed that hydrophobic groups were formed on the surface of specimens by ion N_2 ion irradiation, and the hydrophilic groups were identified as C=O, C-OH, CO_3, and NH_2. The adhesion force of DNA probe to the surface of specimen was increase seven times than untreated by 4 times irradiation of N_2 ion.
社団法人日本機械学会の論文
2006-11-24

327 プラスチック製DNAチップの高性能化の基礎研究(OS-16 微細加工と表面機能)

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