微小重力刺激をうけた艀卵後期の鶏胚における心拍ゆらぎ解析

概要

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The aim of this research is to investigate effects of exposure to microgravity on heart rate fluctuations during last-half incubation of chick embryos. We designed the two experimental groups ; embryos of normal incubation without rotation were a control group, embryos exposed to microgravity by clinostat with velocity of 5 rotations/min for an hour in every incubation days were a rotation group. Instantaneous heart rate (IHR) was determined from the electrocardiogram from day-13 of incubation.to hatch in both groups. In 7 individuals of 11 measurements, although baseline HR decreased about 48 beats/min (averages) at just finished exposure to microgravity, baseline HR recovered during less than 30 min with dependence on embryonic developments. This result indicates that regulation to rotation stimulus involved in cardiovascular system after days-13 of chick embryos is enough developed and probably, younger embryos have strong regulation to microgravity. Although the other 4 embryos died on days-13 and days-18, whether their causes of death related to microgravity or not isn't elucidated. On the other hand, distinctive fluctuations of IHR after exposure to microgravity were not found. In the further research, HR measurements while exposure to microgravity with various rotations (i.e. 3 rpm, 10 rpm) should be required.
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