画像処理によるニワトリ初期胚の瞬時心拍数解析

概要

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The purposes of this research are 1) to achieve long-term recordings of the video images in the early stages of chick embryos and 2) to improve the calculation method of the instantaneous heart rate (IHR) using video images. The video images were captured by CCD camera through a hole opened in the eggshell over an air cell. We succeeded to capture chick embryo from 72 hours of incubation. The amount of the heart movements was calculated from different images of adjacent flames and IHR was calculated from its peak-peak intervals. In order to reduce the incorrect calculation of the IHR due to two peaks in the amount of heart movements at the one beat, we extracted the range of around the atrium or the ventricle images to detect only one peak in a beat. We succeeded to develop the direct and long-term measurement system of the chick embryos or the only its heart and calculation system of the IHR from video images with high accuracy. To investigate cardiac development of the chick embryos in detail, we have to attempt more early stages recordings such as from 48h, in the further study.