画像処理におけるヒヨコの移動量解析システムの構築

概要

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The purposes of this research are 1) to investigate a growth of the cardiovascular system by movies capturing the embryos directly and 2) to develop the calculation system of instantaneous heart rate (IHR) from the captured movies during early stages of chick embryos. The direct monitoring system of chick embryos by a color CCD camera with an image resolution of 680×480 pixels and a sampling rate of 30 frames/sec was build up. The explicit heart beats by tubular heart (1 atrium and 1 ventricle) were clearly observed from 50 hours of incubation at the earliest time. After simple difference images were made from adjacent frames, adverse effects by flickers were eliminated by setting a threshold with subsequent binarization of pixel. This procedure depicted as moving part of the heart in white and motionless part in black. The total number of white pixels in the difference frame was counted as the amount of heart movement which indicates instantaneous changes of heart volume. In order to calculate IHR with high accuracy from peak-peak intervals, we restored time intervals of volume change data to 3000 Hz by interpolation using a sinc function. More long-term measurements and additional experiments should be required in the further study.
函館工業高等専門学校の論文