ニワトリ胚における正常孵化胚と死亡胚の心拍ゆらぎ解析

概要

論文の詳細を見る
In this study, in order to investigate the patterns of heart rate fluctuation (HRF) in both hatched embryos and un-hatched embryos, heart rate (HR) was measured during the hatching period in the chick embryos. Instantaneous heart rate (IHR) was recorded after calculation from ECG waves from day-18 of incubation to hatching. The frequency components which are indices of autonomic nervous activities and the gradient of the spectrum a were determined from the spectrum analysis of HRF. As a result, the distinct pattern of HR-deceleration (HRD) was observed frequently (about 1〜3h) until the external pipping (EP) in the hatched embryos. In contrast, though HRD pattern also occurred in one un-hatched individual, its HR was more decreased than hatched embryo and its appearance become frequent toward embryonic death. Furthermore, although a decrease of a due to internal pipping (IP) was observed, an increase of a accompanying with EP was not found in this un-hatched embryo. Thus, the abnormality involved in cardiovascular and/or respiration system could occur in this un-hatched embryo. In future study, HRD patterns in both hatched embryo and un-hatched embryo should be examined in detail.