Action Pattern of Aeromonas hydrophila Chitinase on Partially N-Acetylated Chitosan(Biological Chemistry)

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概要

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• Oligosaccharides from the digestion of 34% N-acetylated chitosan by Aeromonas hydrophila chitinase were separated by CM-Sephadex C-25 column chromatography. Sugar compositions and the sequences of main oligosaccharides were identified through their N-acetylation, their cleavage with exo-glycosidases, and their degradation with nitrous acid. Hetero-chitooligosaccharides such as GlcN・GlcNAc, GlcN・GlcNAc-GlcN Ac, GlcNAc・GlcN・GlcNAc, and GlcNAc・GlcN・GlcNAc・GlcNAc, together with GlcNAc and (GlcNAc)_2, were detected. The structure of GlcN・GlcNAc was confirmed by the analysis with proton and carbon NMR spectroscopy. These studies indicate that Aeromonas hydrophila chitinase is more specific toward the N-acetyl-β-D-glucosaminidic bonds in partially N-acetylated chitosan.

• 社団法人日本農芸化学会の論文
• 1990-04-23

著者

• MITSUTOMI Masaru

  Department of Applied Biochemistry and Food Science, Faculty of Agriculture, Saga University

• Fukamizo T

  新潟大学 農学部応用生物化学科

• Fukui T

  Faculty Of Agriculture Kinki University

• Fukamizo Tamo

  Department Of Advanced Bioscience Kinki University

• Goto Sachio

  Department Of Biophysical Chemistry Faculty Of Agriculture Kinki University

• Goto Sachio

  Department Of Biochemistry Kinki University School Of Medicine

• OHTAKARA Akira

  Department of Applied Biological Sciences, Faculty of Agriculture, Saga University

• Mitsutomi Masaru

  Department Of Agricultural Chemistry Faculty Of Agriculture Saga University

• Ohtakara A

  Department Of Applied Biological Sciences Faculty Of Agriculture Saga University

• Ohtakara Akira

  Department Of Agricultural Chemistry Faculty Of Agriculture Saga University

• Fukamizo Tamo

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

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