Purification and Characterization of β-N-Acetylhexosaminidase from Pycnoporus cinnabarinus
スポンサーリンク
概要
- 論文の詳細を見る
β-N-Acetylhexosaminidase (EC 3.2. 1.52) was purified from the culture nitrate of Pycnoporus cinnabarinus to homogeneity by polyaerylamide disc gel electrophoresis. The ratio of β-GlcNAcase activity to β-GalNAcase activity remained constant during the purification process. The molecular weight of this enzyme was estimated to be about 120, 000 by gel nitration, and the isoelectric point was at about pH 5.4. The optimum pH was at 2.2 for pNPGlcNAc and around 3.7 for pNPGalNAc. The enzyme was relatively stable at acid pH range of 2-4 (for 45 hr at 5°C) and below 45°C (for 10 min at pH 2.8). The enzyme hydrolysed chito-oligosaccharides, such as N, N-diacetylchitobiose, N, N, N-triacetylchitotriose and N, N, N, N-tetraacetylchitotetraose.
- 社団法人 日本農芸化学会の論文
著者
-
Ohtakara Akira
Department Of Agricultural Chemistry Faculty Of Agriculture Saga University
-
YOSHIDA Miho
Department of Agricultural Chemistry, Faculty of Agriculture, Saga University
-
MURAKAMI Motoharu
Department of Agricultural Chemistry, Faculty of Agriculture, Saga University
-
IZUMI Takako
Department of Agricultural Chemistry, Faculty of Agriculture, Saga University
関連論文
- Preparation of N-Acetylchitooligosaccharides from Enzymatic Hydrolyzates of Chitosan
- Action Pattern of Bacillus sp. No. 7-M Chitosanase on Partially N-Acetylated Chitosan
- Preparation of Chitooligosaccharide-alditols by Hydrogenation with a Ruthenium Catalyst
- NMR Spectra of Partially Deacetylated Chitotrisaccharides(Biological Chemistry)
- Action Pattern of Aeromonas hydrophila Chitinase on Partially N-Acetylated Chitosan(Biological Chemistry)
- Action Pattern of Streptomyces griseus Chitinase on Partially N-Acetylated Chitosan(Biological Chemistry)
- Action of Microbial Chitinases on Chitosan with Different Degrees of Deacetylation(Biological Chemistry)
- Isolation and Identification of Oligosaccharides Produced from Raffinose by Transgalactosylation Reaction of Thermostable α-Galactosidase from Pycnoporus cinnabarinus (Biological Chemistry)
- Immobilization of Thermostable α-Galactosidase from Pycnoporus cinnabarinus on Chitosan Beads and Its Application to the Hydrolysis of Raffinose in Beet Sugar Molasses
- Immobilization of Thermostable α-Galactosidase from Pycnoporus cinnabarinus on Chitin and Some Properties of the Immobilized Enzyme
- Mode of Hydrolysis of Chito-oligosaccharides with Pycnoporus cinnabarinus β-N-Acetylhexosaminidase: Application of High-Performance Liquid Chromatography
- Purification and Some Properties of Acid β-Galactosidase from Pycnoporus cinnabarinus
- Preparation of D-Glucosamine Oligosaccharides by the Enzymatic Hydrolysis of Chitosan(Biological Chemistry)
- Purification and Characterization of β-N-Acetylhexosaminidase from Pycnoporus cinnabarinus