Action Pattern of Streptomyces griseus Chitinase on Partially N-Acetylated Chitosan(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Oligosaccharides produced during the course of the hydrolysis of 25% N-acetylated chitosan by Streptomyces griseus chitinase were fractionated by CM-Sephadex C-25 and Toyopearl HW-40F column chromatographies. Sugar compositions and sequences of main Oligosaccharides were identified by N-acetylation, exo-splitting with β-GlcNAcase and β-GlcNase, and nitrous acid degradation. In addition to N-acetylated saccharides, GlcNAc, (GlcNAc)_2, and (GlcNAc)_3, hetero-chitooligosaccharides such as GlcN・GlcNAc, GlcN・GlcNAc・GlcNAc, GlcN・GlcN・GlcNAc, GlcN・GlcNAc・GlcNAc・GlcNAc, GlcNAc・GlcN・GlcNAc・GlcNAc, GlcN・GlcNAc・GlcN・GlcNAc, and GlcN・GlcN・GlcNAc・GlcNAc were identified. These results indicate that Streptomyces griseus chitinase specifically cleaves the β-acetyl-β-D-elucosaminidic linkages in partially N-acetylated chitosan.
著者
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OHTAKARA Akira
Department of Applied Biological Sciences, Faculty of Agriculture, Saga University
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Mitsutomi Masaru
Department Of Agricultural Chemistry Faculty Of Agriculture Saga University
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Ohtakara Akira
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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Ohtakara Akira
Department Of Agricultural Chemistry Faculty Of Agriculture Saga University
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MATSUNAGA Hirohide
Department of Applied Biological Sciences, Faculty of Agriculture, Saga University
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Matsunaga Hirohide
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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