<Originals>Hypothesis for Mechanism of Action of Enzymes

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概要

• 論文の詳細を見る

• A new hypothesis for the mechanism of action of enzymes is presented. The basic conception of the hypothesis is as follows : (1) there are at least two binding sites at separate part of each enzyme molecule, (2) one is a so-called active site and the other is termed as a "functional site", (3) binding of "functionant" at the functional site is essential for enzymatic action to take place, (4) each binding and releasing phase at the active or the functional site results in a conformational change of the enzyme molecule, and (5) the rate of these conformational changes is much slower than that of binding and releasing steps. Corresponding rate equations are derived. The hypothesis can explain the mechanism by which an enzyme converts a substrate to a product, followed by releasing of the latter from the surface of the enzyme. Applications of this hypothesis to some hydrolytic enzymes are discussed. The probable amino acid residues responsible for the functional sites of these enzymes are postulated on the basis of the three-dimensional structures as well as the chemical and kinetic properties of the enzymes.

• 近畿大学の論文
• 1979-09-28

著者

• Goto Sachio

  Department Of Biochemistry Kinki University School Of Medicine

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