Purification and Properties of Phenoloxidase from Spinach Leaves(Biological Chemistry)
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概要
- 論文の詳細を見る
A phenoloxidase from spinach leaves was extensively purified to homogeneity. The molecular weight of the purified enzyme was 150,000 by gel filtration, and 40,000 by SDS-polyacrylamide gel electrophoresis. The enzyme contained 2 atoms of copper per 150,000 of molecular weight. The optimum pH of the enzyme action is near 6.5. The enzyme lacks cresolase activity. Km and k_o were measured for some 4-substituted catechols and oxygen. Log k_o values for catechols substituted in position 4 by -NO_2, -CHO, -CH_3, -COOH, or -CH_2CH_2COOH were confirmed to fit Hammett relationships (p-Values for σ_p and σ_m are -3.652 and -4.007, respectively), but not for log Km values. Log (k_o/Km) values for the same substrates were confirmed to have this relationship, but protocatechuic and hydrocaffeic acids deviated from this linearity.
- 社団法人日本農芸化学会の論文
- 1989-08-23
著者
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Yamamoto Toshio
Department Of Oral Function And Anatomy In Okayama University Graduate School Of Medicine Dentistry
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KUDO Shoji
Department of Pulmonary Medicine Infection and Oncology, Nippon Medical School
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ODA Yoshiaki
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University
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Oda Yoshiaki
Department Of Agricultural Chemistry Yamagata University
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KATO Hiroaki
Department of Urology
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TAKADA Yoshihiro
Department of Radiology, Hyogo College of Medicine
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TAKAHASHI NORIYUKI
Department of Pathology, National Institute of Health Sciences
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Isoda Yukihiro
Department Of Agricultural Chemistry Yamagata University
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Kudo S
Tohoku Univ. Miyagi Jpn
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Kudo Shoji
Department Of Pulmonary Medicine Infection And Oncology Nippon Medical School
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Takada Yoshihiro
Department Of Agricultural Chemistry Yamagata University
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Kato Hiroaki
Department Of Agricultural Chemistry Yamagata University
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Takahashi Noriyuki
Department Of Biology School Of Education Waseda University
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Takahashi Noriyuki
Department Of Agricultural Chemistry Yamagata University
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Yamamoto Toshio
Department Of Agricultural Chemistry Yamagata University
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KUDO Shoji
Department of Agricultural Chemistry, Yamagata University
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