Effect of Adriamycin on Cultured Mouse Embryo Myocardial Cells(Pharmacological)

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Adriamycin (ADM) inhibited the beating of cultured mouse embryo myocardial cells dose and time-dependently and subsequently lysed the cells. Fifty percent inhibition of beating cell number was observed when cultures were exposed to 430-520μM ADM for 0.5h, 70-100μM ADM for 4h and 3.5 μM for 36 h. When cultures were exposed to 3.5 μM ^3H-ADM, the radioactivity in the cells reached almost the maximum within 8h, and about 90% was recovered as ADM itself even after 72 h. However, the beating cell number and the beating rate did not appreciably decrease within 8h, then decreased to 78.5 and 67.1% at 24 h, and 5.6 and 5.2% at 72 h, respectively. Lysis of the cells was conspicuous at 48 h or later. It was also supported with the decrL'asc in cellular proteins and the release of lactate dehydrogenase. These results indicate that ADM stimulates lysis of the cytoplasmic membrane following inhibition of beating. When cultures wcre exposed to 70μM ADM for 4h and then incubated in ADM-free medium, about 50% of ADM in cells was relcased within 24h. However, inhibition of beating and lysis of such cells still advanced at abtout the same rate as in continuously exposed cells. ADM also decreased the cellular adenosine triphosphate (ATP) content in the same fashion as antimycin A, which inhibited beating in advance of ATP reduction. These results suggested that the beating state of myocardial cells is a sensitive and reliable parameter in this system for studies on the cardiotoxicity of ADM. The system may be useful for studies not only on ADM but aiso on agents having effects on the heart.
社団法人日本薬学会の論文
1987-01-25

Effect of Adriamycin on Cultured Mouse Embryo Myocardial Cells(Pharmacological)

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