Influence of Coenzyme Q_<10> on Doxorubicin Uptake and Metabolism by Mouse Myocardial Cells in Culture

概要

論文の詳細を見る
In order to test the hypothesis that coenzyme Q_<10> (CoQ_<10>) might prevent myocardial cell uptake of doxorubicin (DX), ^3H-DX uptake in cultured myocardial cells was studied. When cultured myocardial cells were incubated with 3.5μM DX for 24-72h or 140μM DX for 1-4h, 120μM CoQ_<10> added in advance protected their beating from DX toxicity. However, CoQ_<10> had no effect on ^3H-DX uptake by the cells under the above condition (3.5μM DX for 24-72h or 140μM DX for 1-4h). Most of the radioactivity in the cells was recovered as DX itself in both the medium with and without CoQ_<10>. Furthermore, the effects of a CoQ homolog (coenzyme Q_9 (CoQ_9)) or an analog (plastoquinone (PQ_9)) on the best inhibition by DX were compared using this system. It was shown that CoQ_9 prevents, though only partially, the inhibition of the myocardial cell beating by 3.5μM DX. PQ_9 was without effect. It is suggested that the protective effect of CoQ_<10> against DX cardiotoxicity was not caused by inhibition of DX uptake or by alteration of DX metabolism in the cells.
社団法人日本薬学会の論文
1988-04-25