A Comparison of Drug Transport through Cultured Monolayers of Bovine Brain Capillary and Bovine Aortic Endothelial Cells
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概要
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To examine how efficient primary cultured brain capillary endothelial cells are as an in vitro blood-brain barrier (BBB) system with regard to drug transport, we compared bovine brain capillary endothelial cells (BBCEC) with bovine aortic endothelial cells (BAEC). Paracellular and transcellular transport were examined. [^<14>C] Sucrose and fluorescein isothiocyanate-dextran (FITC-dextran) (average molecular weight about 4400 and 71200) were used as indices of paracellular transport. The permeability coefficients of [^<14>C] sucrose and FITC-dextran through BBCEC monolayers were about 3 and 30-40 fold lower than those through BAEC monolayers, respectively. Transcellular transport was examined by means of [^3H] eucine and [^3H] alanine uptake studies. The K_m value (Michaelis constant) of the amino acid uptake was lower in BBCEC than in BAEC. BBCEC had higher alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GTP) activity than BAEC when cultured in vitro. These results suggest that BBCEC are suitable for drug transport studies across the BBB in vitro.
- 1994-10-15
著者
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真弓 忠範
Faculty of Pharmaceutical Sciences
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中川 晋作
Faculty of Pharmaceutical Sciences, Osaka University
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宇都口 直樹
帝京大・薬
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宇都口 直樹
昭和薬科大学
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宇都口 直樹
昭和薬科大学薬剤学研究室
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中川 晋作
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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久保 一義
Faculty of Pharmaceutical Science, Osaka University
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久保 一義
Faculty And Graduate School Of Pharmaceutical Sciences Osaka University
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水口 裕之
Faculty of Pharmaceutical Science, Osaka University
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橋岡 祐子
Faculty of Pharmaceutical Science, Osaka University
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宇都口 直樹
Faculty of Pharmaceutical Science, Osaka University
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橋岡 祐子
Faculty Of Pharmaceutical Science Osaka University
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