Evidence That Lipid Peroxidation by Doxorubicin Does Not Initiate the Beat Inhibition of Cultured Mouse Myocardial Cells(Pharmacological,Chemical)
スポンサーリンク
概要
- 論文の詳細を見る
In order to elucidate the biochemical mechanism by which doxorubicin (DX) induces cumulative dose-dependent cardiomyopathy, the effect of DX on beating and on lipid peroxide level in myocardial cells was investigated by using a mouse myocardial cell culture system. Inhibition by DX of the beating of cultured myocardial cells was dose- and exposure time-dependent. When incubated with DX for 0.5h, the number of beating cells decreased to 74% at 172μM, 52% at 345μM and 37% at 517μM, and the lipid peroxide level in the cells increased significantly at 345μM DX or more. At the low dose of 3.5μM DX, though the number of beating cells decreased to 72% at 24h, the lipid peroxide level in the cells was about the same as that without DX at 24h, and increased to about 1.5 times that of the control at 48h. Under the same conditions as above, 120μM a-tocopherol (Toc) added in advance of the treatment reduced by 86% the lipid peroxidation by DX, but did not protect the beating from DX toxicity. In contrast, 120μM coenzyme Q_<10> (CoQ_<10>) reduced the lipid peroxidation by only 33%, but increased the number of beating cells at 48h from 42% to 68%. We conclude that the lipid peroxidation by DX does not initiate the beat inhibition of cultured myocardial cells, and that the protective effect of CoQ_<10> on beating is also based on biochemical actions other than reduction of the lipid peroxidation.
- 社団法人日本薬学会の論文
- 1987-11-25
著者
-
真弓 忠範
Faculty of Pharmaceutical Sciences
-
真弓 忠範
Faculty Of Pharmaceutical Sciences Osaka University
-
紀氏 健雄
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
-
高橋 京子
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
-
紀氏 健雄
神戸学院大学 薬学部
-
真弓 忠範
Faculty Of Pharmaceutical Sciences Kobe-gakuin University
関連論文
- Studies on Ergothioneine. VIII. Preventive Effects of Ergothioneine on Cadmium-induced Teratogenesis
- Amino Acids and Peptides. XXVI. Laminin-Related Peptide Poly (Ethylene Glycol) Hybrids and Their Inhibitory Effect on Experimental Metastasis
- Amino Acids and Peptides. XXV. Preparation of Fibronectin-Related Peptide Poly(ethylene glycol) Hybrids and Their Inhibitory Effect on Experimental Metastasis
- 21世紀型創薬に思うこと
- Effect of a Monoclonal Anti-sperm Antibody (A-1) on in Vitro Fertilization in the Mouse
- Influence of Coenzyme Q_ on Doxorubicin Uptake and Metabolism by Mouse Myocardial Cells in Culture
- Evidence That Lipid Peroxidation by Doxorubicin Does Not Initiate the Beat Inhibition of Cultured Mouse Myocardial Cells(Pharmacological,Chemical)
- Effect of Adriamycin on Cultured Mouse Embryo Myocardial Cells(Pharmacological)
- 培養心筋細胞の拍動並びにATP量に対するAdriamycin及びCoQ_の作用 : 日本ビタミン学会第36回大会一般研究発表要旨
- 2-II-16 心筋細胞に対するアドリアマイシンの脂質過酸化並びに拍動阻害作用に対するα-Toc及びCoQの効果 : 第35回大会一般研究発表要旨 : 日本ビタミン学会
- Studies on Ergothioneine. XI. Inhibitory Effect on Lipid Peroxide Formation in Mouse Liver
- Studies on Ergothioneine. X. Effects of Ergothioneine on the Hepatic Drug Metabolizing Enzyme System and on Experimental Hepatic Injury in Rats
- Studies on Ergothioneine. VII. Some Effects of Ergothioneine on Glycolytic Metabolism in Red Blood Cells from Rats
- 1-II-3 アドリアマイシンによる心筋細胞呼吸及び拍動阻害並びにCoQの保護効果 : 第34回大会研究発表要旨 : 日本ビタミン学会
- Studies on Ergothioneine. VI. Distribution and Fluctuations of Ergothioneine in Rats
- Studies on Bredinin (II) Effect of Guanine Derivatives on Cytotoxicity and Teratogenicity of Bredinin in Rats
- Quantitative Determination of Ergothioneine in Plasma and Tissues by TLC-Densitometry
- Teratogenic Effects of Bredinin, a New Immunosuppressive Agent, in Rats
- Studies on Ergothioneine. V. Determination by High Performance Liquid Chromatography and Application to Metabolic Research
- Amino Acids and Peptides. XXI. Laminin-Related Peptide Analogs Including Poly(Ethylene Glycol) Hybrids and Their Inhibitory Effect on Experimental Metastasis
- PREPARATION OF [ARG-GLY-ASP]-[AMINO-POLY(ETHYLENE GLYCOL)] HYBRIDS AND THEIR INHIBITORY EFFECT ON EXPERIMENTAL METASTASIS
- Amino Acids and Peptides. XXII. Preparation and Antinociceptive Effect of [D-Ala^2] Leu-Enkephalin-Poly (Ethylene Glycol) Hybrid
- Amino Acids and Peptides. XIX. Preparation of Enkephalin-Poly(ethylene glycol) Hybrid and Evaluation of Its Analgesic Activity
- 生化学からみたCoenzyme Qの作用メカニズム (特集/コエンザイムQ10,α-リポ酸の効果を探る)
- ユビキノンはミクロソームでも生合成される?
- 生体内還元型ユビキノンの生理作用に関する研究 : 定量法ならびに亜細胞画分による生成について : 日本ビタミン学会 : 第39回大会研究発表要旨
- Synthesis and Biological Activities of 2,3-Dimethyl-1,4-benzoquinones Having Alkylthio and Arylthio Side Chains(Medicinal Chemistry,Chemical)
- Kearns-Sayre症候群とユビキノン
- ユビキノール : チトクロームCレダクターゼ部位のユビキノン結合タンパク質
- ユビキノン生合成の調整とcAMP
- α-トコフェロールの細胞内転送タンパク質
- ミトコンドリア電子伝達系のユビキノン結合タンパク質
- 公開講座 遺伝子技術と私達の暮らし
- Inhibition of Succinoxidase and Reduced Nicotinamide Adenine Dinucleotide (NADH) Oxidase by 2,3-Ethylenedioxy-1,4-benzoquinones Having Alkylthio and Arylthio Side Chains
- Effect of Bredinin on Early Embryonic Development in Mice
- Amino Acids and Peptides. XX. Preparation of β-Cyclododecyl Aspartate and Its Application to Synthesis of Fibronectin- and Laminin-Related Peptides
- Inhibition of Mitochondrial Respiratory Chain by Arylthiolated 2,3-Ethylenedioxy-1,4-benzoquinones
- A Comparison of Drug Transport through Cultured Monolayers of Bovine Brain Capillary and Bovine Aortic Endothelial Cells
- Serum levels of coenzyme Q10 and lipids in patients during total parenteral nutrition.