海洋における細菌計数法の比較 : 4'6-diamidino-2-phenylindole (DAPI)計数法versus SYBR Green I計数法

概要

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We investigated the availability of a recentdeveloped nucleic acid stain, SYBR Green I, bacterial counting method. Comparison of bacterial counts made with SYBR Green I and DAPI method was conducted using coastal seawater samples. Bacterial counts with both methods were significantly correlated (r=0.60, n=23). However, the ratio of bacterial counts with SYBR Green I and DAPI method was 2.0±0.5, indicating that the count with SYBR green I method was about twice higher than with DAPI method. Bacteria were intensively stained with SYBR Green I and easier to observe under epifluorescence microscopy than with DAPI method. Although it is difficult to identify the reason for difference of the bacterial counts, our results imply a possible existence of bacteria which cannot be detected with the DAPI method used previously.
横浜国立大学の論文
2001-12-31