Quantitative difference in bacterial abundance determined with each protocol for SYBR Green I and 4'6-diamidino-2-phenylindole (DAPI) methods

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概要

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• Bacterial abundance determined with SYBR Green I and 46-diamidino-2-phenylindole (DAPI) methods were compared using the protocol for each method employing epifluorescence microscopy. This comparison, using seawater samples that were collected from Sagami Bay, revealed that the abundance determined with the SYBR Green I method was on average 1.6 fold greater than that with the DAPI method. A model 2 regression analysis showed the following relationship:Log SYBR Green I=-0.238+1.08 Log DAPI (r2=0.85, n=68, p<0.0001). These results suggest that when the method is switched between the SYBR Green I and DAPI methods, the difference in bacterial abundance determined by the two methods should be considered for a quantitatively consistent assessment of the bacterial biomass in the ocean.

• 日本プランクトン学会、日本ベントス学会の論文
• 2007-02-01

著者

• SHIBATA Akira

  Faculty of Pharmaceutical Sciences, Hokuriku University

• Imai Aki

  Faculty Of Engineering Soka University

• Toda Tatsuki

  Faculty Of Engineering Soka University 1-236 Tangi-cho Hachioji Tokyo 192-8577 Japan

• 戸田 龍樹

  創価大学工学部環境共生工学科

• Toda Tatsuki

  Facuity Of Engineering Soka University

• 戸田 龍樹

  創価大学工学部

• TAGUCHI Satoru

  Faculty of Engineering, Soka University

• KIKUCHI Tomohiko

  Faculty of Education and Human Sciences, Yokohama National University

• HARA SEIKO

  Faculty of Comparative Culture, Miyazaki International College

• TODA TATUKI

  Faculty of Engineering, Soka University

• Shibata Akira

  Univ. Tokyo Tokyo Jpn

• Shibata Akira

  Faculty Of Engineering Soka University

• Hara Seiko

  Faculty Of Comparative Culture Miyazaki International College

• 戸田 龍樹

  創価大学工学部 環境共生工学科

• Taguchi S

  Hokkaido National Fisheries Research Institute

• Taguchi S

  Soka University

• Taguchi Satoru

  Faculty Of Engineering Soka University

• Taguchi S

  Faculty Of Engineering Soka University

• Kikuchi Tomohiko

  Yokohama National Univ. Kanagawa Jpn

• Kikuchi Tomohiko

  Faculty Of Education And Human Sciences Yokohama National University

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