Fate of the bacterial cell envelope component, lipopolysaccharide, that is sequentially mediated by viruses and flagellates
スポンサーリンク
概要
- 論文の詳細を見る
Transformation of a cell envelope component of bacteria, lipopolysaccharide (LPS), from bacterial cells into a dissolved form was investigated during time course experiments using seawater culture in conjunction with determining population dynamics of three microbial groups, which are flagellates, bacteria, and viruses. The surface seawater collected from a coastal site was enriched with glutamic acid, and then incubated. LPS concentration was determined using the Limulus amebocyte lysate assay. In the early period of incubation, LPS in the particulate fraction (>2m μm) increased to the maximum in association with bacterial abundance; thereafter, both decreased together. During the fluctuation of LPS in the particulate fraction, LPS in the dissolved fraction (>2m μ) also increased with viral abundance, but then decreased after flagellate abundance began to increase. However, in a culture condition without flagellates, LPS in both dissolved and particulate fractions increased continuously in association with viral abundance. This increase in LPS in the two fractions associated with viral abundance continued while bacterial abundance was maintained at a similar level between the middle and late incubation periods. The temporal change in LPS in the two fractions and its coupled microbial dynamics indicate that LPS in bacterial cells was transformed into nonliving dissolved and particulate forms by viral lysis and that thereafter the LPS was removed through ingestion by flagellates. Thus, our results suggest that the fate of the bacterial cell envelop component, LPS, that is sequentially mediated by viruses and flagellates in the sea.
- International Coastal Research Center, Ocean Research Institute, the University of Tokyo,東京大学海洋研究所国際沿岸海洋研究センター,Ocean Research Institute, The University of Tokyo, Japan,Faculty of Engineering, Soka University, Japan,Faculty of Education and Human Sciences,の論文
- 2009-08-20
著者
-
Toda Tatsuki
Faculty Of Engineering Soka University 1-236 Tangi-cho Hachioji Tokyo 192-8577 Japan
-
戸田 龍樹
創価大学工学部環境共生工学科
-
Toda Tatsuki
Facuity Of Engineering Soka University
-
戸田 龍樹
創価大学工学部
-
Shibata Akira
Univ. Tokyo Tokyo Jpn
-
戸田 龍樹
創価大学工学部 環境共生工学科
-
Taguchi S
Hokkaido National Fisheries Research Institute
-
Taguchi S
Soka University
-
Taguchi S
Faculty Of Engineering Soka University
関連論文
- Surface zooplankton distribution in the Drake Passage recorded by Continuous Plankton Recorder (CPR) in late austral summer of 2000
- 相模湾真鶴半島周辺海域におけるプランクトンとベントスの研究(2)
- 生ごみ可溶化液を基質としたUASB法による連続処理
- 沿岸域ネットプランクトン試料中に見られた高い灰分含量 : 乾燥重量による動物プランクトン現存量測定の誤差要因
- 海洋における細菌計数法の比較 : 4'6-diamidino-2-phenylindole (DAPI)計数法versus SYBR Green I計数法
- 日本の温帯沿岸域における有光層内での紫外線透過の特性(Journal of Oceanography Vol.56(2000))
- 相模湾真鶴港における海洋環境の周年ならびに経年変化 : 1995年から1999年
- 北海道東部当幌川湿原における水生無脊椎動物相
- P-171 生ごみ処理機から分離した中等度好熱菌の解析(ポスター発表)
- 低真空走査型電子顕微鏡を利用したプランクトン観察のための新しい凍結乾燥法とその応用
- 相模湾,真鶴港内におけるノープリウスy(Crustacea: Maxillopoda: Facetotecta)の分布と季節消長
- Seasonal variability of size-fractionated chlorophyll a in Monbetsu Harbor, Hokkaido, northern Japan
- High Ash Content in Net-Plankton Samples from Shallow Coastal Water: Possible Source of Error in Dry Weight Measuremet of Zooplankton Biomass
- Summer phytoplankton bloom in Manazuru Harbor, Sagami Bay, central Japan
- Variability in the Relative Penetration of Ultraviolet Radiation to Photosynthetically Available Radiation in Temperate Coastal Waters, Japan
- 相模湾真鶴半島周辺海域に出現するカイアシ類(Crustacea, Maxillopoda)
- 相模湾真鶴海岸のモエギイソギンチャクAnthopleura midori UCHIDA & MURAMATSU(Anthozoa: Actiniidae)に寄生するかいあし類について
- Quantitative difference in bacterial abundance determined with each protocol for SYBR Green I and 4'6-diamidino-2-phenylindole (DAPI) methods
- Zooplankton distribution patterns in relation to the Antarctic Polar Front Zones Recorded by Continuous Plankton Recorder (CPR) during 1999/2000 Kaiyo Maru cruise
- Effect of ice algal community on the increase of chlorophyll a concentration during spring in coastal water of the Sea of Okhotsk
- Fate of the bacterial cell envelope component, lipopolysaccharide, that is sequentially mediated by viruses and flagellates
- Seasonal variability in chromophoric dissolved organic matter in the Sakawa River and Sagami Bay, Japan
- The variability in abundance of eugregarines living in the Antarctic krill
- Efficient capture of deep - sea hyperbenthic calanoid copepods with baited traps
- Cnidarians and ctenophores observed from the manned submersible Shinkai 2000 in the midwater of Sagami Bay, Pacific coast of Japan
- Respiration of adult female Calanus hyperboreus (Copepoda) during spring in the North Water Polynya
- Variability in timing and magnitude of spring bloom in the Oyashio region, the western subarctic Pacific off Hokkaido, Japan
- Horizontal distribution and seasonal variability of the epipelagic chaetognath Sagitta elegans in relation to hydrography in the western subarctic Pacific Ocean
- ESTIMATION OF VERTICAL DISTRIBUTION OF CHLOROPHYLL a OFF EAST HOKKAIDO BY GAUSSIAN CURVE FITTING (15th Symposium on Polar Biology)
- EFFECT OF SHAPE OF SEDIMENT TRAP ON MEASUREMENT OF VERTICAL FLUX OF PARTICLES : PRELIMINARY RESULTS (14th Symposium on Polar Biology)
- Usage of seawater system for long-term study of phytoplankton in the boreal coastal water of Hokkaido, Japan
- Reproductive strategy of Acartia steueri in Sagami Bay, Japan
- 動物プランクトンおよび卵の孵化に対する圧力の影響測定用高圧システム
- 培養法と非培養法による好気固相反応型生ごみ処理機における微生物の解析
- 単位プロセスの多機能化による有機性廃棄物の低環境負荷処理技術の開発 (特集 注目される新技術の実用化)
- 異なる充填材を用いた好気固相反応型生ごみ処理槽における微生物相の解析
- 深海プランクトンに関する最近の研究 (特集 深海)
- Calanidae科かいあし類の分類に関する諸問題
- Modeling of Spring Bloom in the Western Subarctic Pacific (off Japan) with Observed Vertical Density Structure
- 相模湾深海底冷湧水域の近底層動物プランクトン〔英文〕
- Euphausia属の胃咀嚼器の形態(甲殻綱,オキアミ目)〔英文〕