アフェレシス療法における温度変化を利用した分画分離技術(<特集>アフェレシス療法における技術的進歩 : 最近のトピックスから)

概要

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Cryofiltration utilizes a cooling technique in plasma fractionation to increase separation efficiency between the albumin and globulin fractions by utilizing the aggregation property of the globulins. On the other hand, DF thermo utilizes a heating technique to keep the temperature in plasma fractionation around 37℃ to improve the separation characteristics of the plasma fractionator due to the decreasing degree of membrane fouling. However, the effect of temperature on the separation property of the membrane has not yet been clarified. An in vitro study using pooled human plasma harvested by several DFPP treatments for a patient was carried out in this project to examine the relationship between temperature and separation efficiency in a plasma fractionator, Evaflux 5 A, Kuraray Medical Company Limited. As a result, improvement of plasma fractionation between albumin and globulin related to the toxin was observed through the increasing temperature of the plasma fractionator. Although the albumin concentration in the filtrate in the plasma fractionator decreased with time through membrane fouling due to protein deposition, its tendencies were remarkable in the lower temperature in plasma fractionation. Heating to around 37℃ is effective to retain the separation property of the membrane in the plasma fractionator in comparison with conventional DFPP treatment.
日本アフェレシス学会の論文
2006-05-31