担LNCaP腫瘍ヌードマウスにおける^<131>I標識抗γ-seminoproteinモノクローナル抗体および^<67>Ga-citrateを用いた腫瘍イメージング

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The gamma camera images of nude mice bearing xenografts of a human prostatic adenocarcinoma cell line LNCaP were evaluated after intravenous administration of ^<131>I-labeled monoclonal antibody to γ-seminoprotein(γ-Sm) or ^<67>Ga-citrate. The monoclonal antibody to γ-Sm (murine IgG_1, K) was radiolabeled with iodine-131 by lactoperoxidase method followed by separation of free ^<131>I with membrane ultrafiltration method (^<131>I-GSM). The ^<131>I-GSM was injected intravenously (5.54 MBq/27.7μg) in two nude mice, and 2MBq of ^<67>Ga-citrate in a nude mouse. Radioactive uptake by LNCaP xenografts was compared between two scanning agents on sequential scintigrams. With ^<131>I-GSM, optimal images of the tumor were provided at 4 to 7 days after administration. With ^<67>Ga-citrate, visualization of the tumor was enabled but background radioactivity was high, making it difficult to distinguish the margin of the tumor from normal organ tissue. Moreover, in the tumor visualized by ^<67>Ga-citrate the radioactivity decreased notably on the second day. Compared with ^<67>Ga-citrate, ^<131>I-GSM enabled sufficient contrast between the background and the tumor, and provided a longer visualization period. These results show that immunoscintigraphy with radiolabeled monoclonal antibody to γ-Sm is a feasible and promising study for improvement of diagnostic modality of adenocarcinoma of the prostate.
北里大学の論文
1994-02-28

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