Localization of RANKL and cathepsin K, B and L in rat periodontal tissues during experimental tooth movement

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The differentiation and functions of osteoclasts are regulated by osteoblast-derived factors such as receptor activator of NFκB ligand (RANKL) that stimulates osteoclasts formation. The role of cathepsin K (CAK), cloned as a novel collagenolytic cysteine proteinase, cathepsin L (CAL) and cathepsin B (CAB) in bone resorption was investigated. A recent immunocytochemical study reported that CAB and CAL were localized in the periodontal ligament (PDL) of rat molars. The present study focused on the localization of RANKL, CAB, CAL and CAK during experimental tooth movement in rats, and then elucidates mechanism of tooth movement. Experimental tooth movement was accomplished for 1, 3 and 7 days using the Waldo method. To determine localization of RANKL, CAB, CAL and CAK proteins using immunohistochemistry. Immunoreactivity for RANKL became strong in osteoblasts, osteoclasts and PDL fibroblasts from 1 day after tooth movement. Further, CAB and CAL were detected in osteoclasts and PDL fibroblasts and CAK was detected in osteoclasts specifically. On 3 days after tooth movement, immunoreactivity of those cathepsins was increased in osteoclasts and fibroblasts. The present study results showed that immunoreactivity for RANKL became stronger with tooth movement. Further, cathepsins expression was increased in osteoclasts and fibroblasts later than RANKL expression. Therefore, RANKL, CAB, CAL and CAK may be involved in the process of bone resorption during tooth movement.
日本矯正歯科学会の論文

Localization of RANKL and cathepsin K, B and L in rat periodontal tissues during experimental tooth movement

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