酵素免疫測定法におけるOne-step標識法の評価

概要

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We evaluated two different one-step enzyme labeling methods for murine monoclonal thyroglobulin(Tg) antibodies with horseradish peroxidase (HRP). In these methods, unlabeled Tg monoclonal antibodies were labeled with HRP-conjugated anti-mouse IgG or anti-mouse Fab. These procedures were compared with the conventional periodate labeling method with HRP. The one-step labeling methods gave excellent results in measuring serum Tg by enzyme immunoassay and by the enzyme inhibition test. In addition, the one-step enzyme labeling procedures are simple to perform and take only 15 minutes to complete, while the conventional periodate labeling procedure takes almost two full days. The one-step labeling method would be useful for characterization of monoclonal antibodies in enzyme immunoassay.
香川県立保健医療大学の論文
2004-03-31