水田におけるアキアカネ幼虫のシナハマダラカ幼虫に対する天敵としての役割に関する研究 : 1. 捕食率推定における免疫電気浸透法 (Electrosyneresis) 利用についての検討

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The precipitin test using the electrosyneresis (ES method) was evaluated for the purpose of detecting the mosquito larvae (An. sinensis) from the gut of the dragonfly nymphs (Sympetrum frequens), in the course of studies for their prey-predator relationships in rice fields. Antiserum was obtained from rabbits immunized by intramuscular injections of saline extracts of An. sinensis (4th instar larvae). This antiserum showed the strong cross-reaction between An. sinensis and Cx. tritaeniorhynchus, and Chironomus sp. which might be fed by the dragonfly nymphs in the census fields. The specific antiserum to An. sinensis larvae was prepared by absorbing the antiserum with the saline extracts of Cx. tritaeniorhynchus. Using the specific antiserum on ES method, it was experimentally confirmed that the 4th instar larva of An. sinensis in the gut of the 9th instar nymphs of Sympetrum frequens could be detected with confidence up to 33hr after feeding in all cases of trials and up to 54hr in one case. It was concluded that the ES method could be useful and appliable to the quantitative study of their prey-predator relationships.
1982-03-15