スピンイムノアッセイによる尿中遊離型コルチゾールの定量
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概要
- 論文の詳細を見る
A spin-labeled derivative of cortisol was prepared by coupling cortisol-3-(O-carboxylmethyl)oxime with 3-amino-2,2,5,5-tetramethyl-1-oxypyrrolidine and used to develop a method for measuring free cortisol in the human urine. The samples were extracted by ethyl ether and measured by spin immunoassay (SIA) both with and without a preceding thin-layer chromatography (TLC). Although the values obtained from SIA without TLC purification were higher than those with TLC, the relationship between them was fairly good (r=0.980). Cortisol levels were determined in eleven normal urine samples simultaneously by SIA and radioimmunoassay without TLC purification. The coefficient of correlation was 0.988. The method was found to be speedy and simple, and to be useful for the routine use.
- 公益社団法人日本薬学会の論文
- 1983-05-25
著者
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細川 幹夫
Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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佐用 博照
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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細川 幹夫
神戸学院大学薬学部
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