Determination of Phenobarbital in Human Serum by Spin Immunoassay
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概要
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A deuterated spin-labeled derivative of phenobarbital was prepared by coupling 5-ethyl-5-phenylbarbituryl-1-butyric acid (PB-BUA) with 4-amino-1-oxyl-2,2,6,6-tetramethylpiperidine-d_<17> and used to develop a method for measuring phenobarbital levels in human serum. Antiserum was raised in rabbits immunized with the PB-BUA/bovine serum albumin conjugate. With 2μl of serum, the sensitivity limit of the assay was 10ng of phenobarbital. Phenobarbital levels in QPAK therapeutic drug-monitoring control sera were determined by spin immunoassay (SIA). The values obtained by SIA were in good agreement with those presented by the manufacturer. The method was found to be simple and suitable for routine use, though one of the expected advantages of SIA, i.e., rapidity of analysis, was not realized.
- 公益社団法人日本薬学会の論文
- 1984-09-25
著者
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細川 幹夫
Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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佐用 博照
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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細川 幹夫
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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佐用 博照
Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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