The Effect of Alkaline Denaturation on Organic Hydroperoxide-supported N-Demethylase Activities of Catalase
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概要
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The enzymic activities of various preparations of catalase were determined. Catalase C-100,which is a twice-crystallized preparation, showed the highest catalatic activity, whereas C-40,which is a lyophilized preparation, showed the highest N-demethylase activities towards aminopyrine. The ethyl hydroperoxide (EHP)-supported peroxidatic activity towards methanol increased in parallel with the increase in the catalatic activity. On the other hand, the order of increasing N-demethylase activities towards aminopyrine was not the same as that of increasing catalatic activity. Treatment with alkali followed by neutralization increased the N-demethylase activities of C-40 and C-100 and decreased both the peroxidatic activity towards methanol and the catalatic activity. When the alkali-treated catalase solution was neutralized and allowed to reconstitute, the increased levels of N-demethylase activities only slightly decreased with time up to 2 hr. The decreased peroxidatic activity towards methanol was restored nearly completely, whereas only 7.6% of the original catalatic activity of C-40 was restored after reconstitution for 24 hr. Gel chromatographic experiments showed that treatment with alkali results in the dissociation of catalase into subunits, and that the recombination of the subunits takes place fairly rapidly on neutralization of the alkali-treated catalase. The present results suggest that catalase molecules with a loosely combined quaternary structure, which are formed by lyophilization or by treatment with alkali followed by neutralization, are capable of catalyzing the organic hydroperoxide-supported N-demethylation of aminopyrine, whereas they cannot decompose hydrogen peroxide efficiently.
- 公益社団法人日本薬学会の論文
- 1981-01-25
著者
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細川 幹夫
Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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佐用 博照
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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細川 幹夫
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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佐用 博照
Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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