Results of Spin Immunoassay for Simultaneous Measurement of Phenytoin and Phenobarbital in Serum Compared with Those of Liquid Chromatography
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概要
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We describe a spin immunoassay (SIA) in which the two antiepileptic agents, phenytoin (PHT) and phenobarbital (PB), are determined simultaneously in a single measurement. The method involves labeling the two with ^<15>N-and ^<14>N-nitroxide, respectively, whose electron spin resonance peaks do not overlap each other. With 2μl of serum, the smallest detectable concentration for either drug was 1.25 mg/l. PHT and PB levels in Q-PAK therapeutic drug-monitoring control sera were determined by SIA. The values obtained by SIA were in good agreement with those presented by the manufacturer. Sera from rabbits after administration of PHT and/or PB were analyzed by SIA and high-performance liquid chromatography. There was a good correlation between results obtained by the two methods.
- 公益社団法人日本薬学会の論文
- 1985-07-25
著者
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細川 幹夫
Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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佐用 博照
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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細川 幹夫
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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佐用 博照
Faculty Of Pharmaceutical Sciences Kobe-gakuin University
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